Respiratory gas exchange, nitrogenous waste excretion, and fuel usage during aerobic swimming in juvenile rainbow trout

R. F. Lauff, C. H. Wood

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

The types of fuel burned by juvenile rainbow trout (17 g) during a 58-h period of aerobic sustained exercise were studied by respirometry. Attempts to measure fuel usage by depletion (the compositional approach) in these same fish were unsuccessful due to lack of detectable changes in proximate body composition. O2 consumption CO2 excretion, and nitrogenous waste excretion (ammonia-N plus urea-N) were measured in individual fish swum continuously at 55% and 80% of maximum sustainable swimming speed and in non-swimming controls. O2 consumption and CO2 excretion increased with swimming speed, and decreased over time. Absolute rates of N exertion were independent of swimming speed and time. Instantaneous aerobic fuel use, as calculated from the respiratory quotients and nitrogen quotients, was approximately 47% lipid, 30% protein, and 23% carbohydrate in non-swimmers at the start of the experiment. With increased swimming speed there was no change in absolute rates of protein oxidation, while lipid and carbohydrate oxidation both increased. Therefore, the relative protein contribution decreased with increasing speed but increased with swimming duration as the oxidation of other fuels declined over time. However, lipid oxidation predominated at all speeds and at all times. The relative contribution of carbohydrate increased with swimming speed and decreased over time. These results suggest that swimming becomes more efficient over time and help resolve uncertainties in the literature. We conclude that lipid is the main fuel of aerobic exercise, that protein catabolism is kept at minimum levels necessary for maintenance, and that carbohydrate oxidation becomes more important with increased white muscle recruitment at higher speed.

Original languageEnglish
Pages (from-to)501-509
Number of pages9
JournalJournal of Comparative Physiology - B Biochemical, Systemic, and Environmental Physiology
Volume166
Issue number8
DOIs
StatePublished - Dec 1 1996

Fingerprint

pulmonary gas exchange
respiratory gases
Oncorhynchus mykiss
gas exchange
excretion
rainbow
Gases
carbohydrate
Carbohydrates
oxidation
Oxidation
carbohydrates
lipid
Lipids
protein
Fishes
Fish
Proteins
exercise
lipid peroxidation

Keywords

  • Carbohydrate
  • Fuel
  • Lipid
  • Nitrogen quotient
  • Protein
  • Rainbow trout
  • Respiratory quotient
  • Swimming

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Physiology
  • Environmental Science(all)
  • Physiology (medical)

Cite this

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abstract = "The types of fuel burned by juvenile rainbow trout (17 g) during a 58-h period of aerobic sustained exercise were studied by respirometry. Attempts to measure fuel usage by depletion (the compositional approach) in these same fish were unsuccessful due to lack of detectable changes in proximate body composition. O2 consumption CO2 excretion, and nitrogenous waste excretion (ammonia-N plus urea-N) were measured in individual fish swum continuously at 55{\%} and 80{\%} of maximum sustainable swimming speed and in non-swimming controls. O2 consumption and CO2 excretion increased with swimming speed, and decreased over time. Absolute rates of N exertion were independent of swimming speed and time. Instantaneous aerobic fuel use, as calculated from the respiratory quotients and nitrogen quotients, was approximately 47{\%} lipid, 30{\%} protein, and 23{\%} carbohydrate in non-swimmers at the start of the experiment. With increased swimming speed there was no change in absolute rates of protein oxidation, while lipid and carbohydrate oxidation both increased. Therefore, the relative protein contribution decreased with increasing speed but increased with swimming duration as the oxidation of other fuels declined over time. However, lipid oxidation predominated at all speeds and at all times. The relative contribution of carbohydrate increased with swimming speed and decreased over time. These results suggest that swimming becomes more efficient over time and help resolve uncertainties in the literature. We conclude that lipid is the main fuel of aerobic exercise, that protein catabolism is kept at minimum levels necessary for maintenance, and that carbohydrate oxidation becomes more important with increased white muscle recruitment at higher speed.",
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N2 - The types of fuel burned by juvenile rainbow trout (17 g) during a 58-h period of aerobic sustained exercise were studied by respirometry. Attempts to measure fuel usage by depletion (the compositional approach) in these same fish were unsuccessful due to lack of detectable changes in proximate body composition. O2 consumption CO2 excretion, and nitrogenous waste excretion (ammonia-N plus urea-N) were measured in individual fish swum continuously at 55% and 80% of maximum sustainable swimming speed and in non-swimming controls. O2 consumption and CO2 excretion increased with swimming speed, and decreased over time. Absolute rates of N exertion were independent of swimming speed and time. Instantaneous aerobic fuel use, as calculated from the respiratory quotients and nitrogen quotients, was approximately 47% lipid, 30% protein, and 23% carbohydrate in non-swimmers at the start of the experiment. With increased swimming speed there was no change in absolute rates of protein oxidation, while lipid and carbohydrate oxidation both increased. Therefore, the relative protein contribution decreased with increasing speed but increased with swimming duration as the oxidation of other fuels declined over time. However, lipid oxidation predominated at all speeds and at all times. The relative contribution of carbohydrate increased with swimming speed and decreased over time. These results suggest that swimming becomes more efficient over time and help resolve uncertainties in the literature. We conclude that lipid is the main fuel of aerobic exercise, that protein catabolism is kept at minimum levels necessary for maintenance, and that carbohydrate oxidation becomes more important with increased white muscle recruitment at higher speed.

AB - The types of fuel burned by juvenile rainbow trout (17 g) during a 58-h period of aerobic sustained exercise were studied by respirometry. Attempts to measure fuel usage by depletion (the compositional approach) in these same fish were unsuccessful due to lack of detectable changes in proximate body composition. O2 consumption CO2 excretion, and nitrogenous waste excretion (ammonia-N plus urea-N) were measured in individual fish swum continuously at 55% and 80% of maximum sustainable swimming speed and in non-swimming controls. O2 consumption and CO2 excretion increased with swimming speed, and decreased over time. Absolute rates of N exertion were independent of swimming speed and time. Instantaneous aerobic fuel use, as calculated from the respiratory quotients and nitrogen quotients, was approximately 47% lipid, 30% protein, and 23% carbohydrate in non-swimmers at the start of the experiment. With increased swimming speed there was no change in absolute rates of protein oxidation, while lipid and carbohydrate oxidation both increased. Therefore, the relative protein contribution decreased with increasing speed but increased with swimming duration as the oxidation of other fuels declined over time. However, lipid oxidation predominated at all speeds and at all times. The relative contribution of carbohydrate increased with swimming speed and decreased over time. These results suggest that swimming becomes more efficient over time and help resolve uncertainties in the literature. We conclude that lipid is the main fuel of aerobic exercise, that protein catabolism is kept at minimum levels necessary for maintenance, and that carbohydrate oxidation becomes more important with increased white muscle recruitment at higher speed.

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