Resonance energy transfer methods of RNA detection

Kyle A. Cissell, Eric A. Hunt, Sapna K. Deo

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Quantitation of RNA is important in diagnostics, environmental science, and basic biomedical research. RNA is considered a signature for pathogen identification, and its expression profile is linked with disease pathogenesis, allowing for biomarker identification. RNA-based diagnostics is an emerging field of research. This expansion of interest in studying RNA has generated demand for its accurate and sensitive detection. Several methods have therefore been developed to detect RNA. Resonance energy transfer methods of RNA detection are highly promising in terms of simplicity and high sensitivity. In this review, we have focused on the latest developments in resonance energy transfer methods of RNA detection that utilize various probe designs. The probe designs discussed here are molecular beacons, quenched autoligation probes, and linear oligonucleotide probes. Resonance energy transfer methods based on both fluorescence and bioluminescence detection are discussed.

Original languageEnglish (US)
Pages (from-to)125-135
Number of pages11
JournalAnalytical and bioanalytical chemistry
Volume393
Issue number1
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

Keywords

  • Bioluminescence resonance energy transfer
  • Fluorescence resonance energy transfer
  • Molecular beacons
  • Quenched autoligation probes
  • RNA detection

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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