Resolution of Aminoacyl Transfer Ribonucleic Acid by Hydroxylapatite Chromatography

Karl H. Muench, Paul Berg

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Aminoacyl ribonucleic acid from E. coli has been resolved on columns of hydroxylapatite with gradient elution by potassium phosphate buffers, pH 5.8. Transfer ribonucleic acid esterified to alanine, leucine, methionine, and valine is fractionated into multiple peaks. After prior purification by partition chromatography, individual t-RNA species have been resolved to 80-90% purity by hydroxylapatite. The acyl bonds are stable enough to permit recovery of intact aminoacyl RNA from column effluents, facilitating analysis. Column capacity is at least 24 mg of t-RNA/cm2 of column cross-sectional area. Recoveries of t-RNA and of acceptor activities exceed 90%.

Original languageEnglish (US)
Pages (from-to)982-987
Number of pages6
JournalBiochemistry
Volume5
Issue number3
DOIs
StatePublished - Mar 1 1966

ASJC Scopus subject areas

  • Biochemistry

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