Resistance of Yersinia pestis to complement-dependent killing is mediated by the ail outer membrane protein

Sara Schesser Bartra, Katie L. Styer, Deanna M. O'Bryant, Matthew L. Nilles, B. Joseph Hinnebusch, Alejandro Aballay, Gregory V Plano

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Yersinia pestis, the causative agent of plague, must survive in blood in order to cause disease and to be transmitted from host to host by fleas. Members of the Ail/Lom family of outer membrane proteins provide protection from complement-dependent killing for a number of pathogenic bacteria. The Y. pestis KIM genome is predicted to encode four Ail/Lom family proteins. Y. pestis mutants specifically deficient in expression of each of these proteins were constructed using lambda Red-mediated recombination. The Ail outer membrane protein was essential for Y. pestis to resist complement-mediated killing at 26 and 37°C. Ail was expressed at high levels at both 26 and 37°C, but not at 6°C. Expression of Ail in Escherichia coli provided protection from the bactericidal activity of complement. High-level expression of the three other Y. pestis Ail/Lom family proteins (the y1682, y2034, and y2446 proteins) provided no protection against complement-mediated bacterial killing. A Y. pestis ail deletion mutant was rapidly killed by sera obtained from all mammals tested except mouse serum. The role of Ail in infection of mice, Caenorhabditis elegans, and fleas was investigated.

Original languageEnglish
Pages (from-to)612-622
Number of pages11
JournalInfection and Immunity
Volume76
Issue number2
DOIs
StatePublished - Feb 1 2008

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Yersinia pestis
Membrane Proteins
Siphonaptera
Proteins
Plague
Caenorhabditis elegans
Serum
Genetic Recombination
Mammals
Genome
Escherichia coli
Bacteria
Infection

ASJC Scopus subject areas

  • Immunology

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Resistance of Yersinia pestis to complement-dependent killing is mediated by the ail outer membrane protein. / Bartra, Sara Schesser; Styer, Katie L.; O'Bryant, Deanna M.; Nilles, Matthew L.; Hinnebusch, B. Joseph; Aballay, Alejandro; Plano, Gregory V.

In: Infection and Immunity, Vol. 76, No. 2, 01.02.2008, p. 612-622.

Research output: Contribution to journalArticle

Bartra, Sara Schesser ; Styer, Katie L. ; O'Bryant, Deanna M. ; Nilles, Matthew L. ; Hinnebusch, B. Joseph ; Aballay, Alejandro ; Plano, Gregory V. / Resistance of Yersinia pestis to complement-dependent killing is mediated by the ail outer membrane protein. In: Infection and Immunity. 2008 ; Vol. 76, No. 2. pp. 612-622.
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