Requirement of dimerization for RNA editing activity of adenosine deaminases acting on RNA

Dan Sung C Cho, Weidong Yang, Joshua T. Lee, Ramin Shiekhattar, John M. Murray, Kazuko Nishikura

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Adenosine deaminases acting on RNA (ADAR) convert adenosine residues into inosines in double-stranded RNA. Three vertebrate ADAR gene family members, ADAR1, ADAR2, and ADAR3, have been identified. The catalytic domain of all three ADAR gene family members is very similar to that of Escherichia coli cytidine deaminase and APOBEC-1. Homodimerization is essential for the enzyme activity of those cytidine deaminases. In this study, we investigated the formation of complexes between differentially epitope-tagged ADAR monomers by sequential affinity chromatography and size exclusion column chromatography. Both ADAR1 and ADAR2 form a stable enzymatically active homodimer complex, whereas ADAR3 remains as a monomeric, enzymatically inactive form. No heterodimer complex formation among different ADAR gene family members was detected. Analysis of HeLa and mouse brain nuclear extracts suggested that endogenous ADAR1 and ADAR2 both form a homodimer complex. Interestingly, endogenous ADAR3 also appears to form a homodimer complex, indicating the presence of a brain-specific mechanism for ADAR3 dimerization. Homodimer formation may be necessary for ADAR to act as active deaminases. Analysis of dimer complexes consisting of one wild-type and one mutant monomer suggests functional interactions between the two subunits during site-selective RNA editing.

Original languageEnglish (US)
Pages (from-to)17093-17102
Number of pages10
JournalJournal of Biological Chemistry
Volume278
Issue number19
DOIs
StatePublished - May 9 2003
Externally publishedYes

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RNA Editing
Adenosine Deaminase
Dimerization
RNA
Cytidine Deaminase
Genes
Brain
Monomers
Inosine
Double-Stranded RNA
Affinity chromatography
Column chromatography
Size exclusion chromatography
Affinity Chromatography
Adenosine
Enzyme activity
Gel Chromatography
Vertebrates
Epitopes
Catalytic Domain

ASJC Scopus subject areas

  • Biochemistry

Cite this

Requirement of dimerization for RNA editing activity of adenosine deaminases acting on RNA. / Cho, Dan Sung C; Yang, Weidong; Lee, Joshua T.; Shiekhattar, Ramin; Murray, John M.; Nishikura, Kazuko.

In: Journal of Biological Chemistry, Vol. 278, No. 19, 09.05.2003, p. 17093-17102.

Research output: Contribution to journalArticle

Cho, Dan Sung C ; Yang, Weidong ; Lee, Joshua T. ; Shiekhattar, Ramin ; Murray, John M. ; Nishikura, Kazuko. / Requirement of dimerization for RNA editing activity of adenosine deaminases acting on RNA. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 19. pp. 17093-17102.
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