Reproducible expansion and characterization of mouse neural stem/progenitor cells in adherent cultures derived from the adult subventricular zone

Michelle H. Theus, Jerome Ricard, Daniel J Liebl

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Endogenous neural stem/progenitor cells (NSPCs) residing in the subventricular zone (SVZ) of the adult mouse forebrain have been shown to enhance their neurogenic potential in response to CNS injury. Mechanisms involved in regulating adult neurogenesis under naïve or stressed conditions can be studied using a monolayer cell-culture system of the nestin-expressing NSPC lineage to analyze proliferation, survival, and differentiation. Here, a protocol for the expansion of NSPCs for studies aimed at understanding the functional role of NSPCs in maintaining adult neurogenic processes is described. This unit outlines detailed procedures for: (1) isolation, maintenance, and culture of the NSPC component of the SVZ niche from the lateral wall of the lateral ventricle; (2) characterization of NSPC functions by examining proliferation, survival, and differentiation; and (3) efficient siRNA transfection methods in 96-well format.

Original languageEnglish
Article number2D.8
JournalCurrent Protocols in Stem Cell Biology
Volume1
Issue numberSUPPL.20
DOIs
StatePublished - Mar 1 2012

Fingerprint

Neural Stem Cells
Lateral Ventricles
Stem Cells
Nestin
Neurogenesis
Cell Lineage
Cellular Structures
Survival Analysis
Prosencephalon
Small Interfering RNA
Transfection
Cell Culture Techniques
Maintenance
Wounds and Injuries

Keywords

  • Cultures
  • Differentiation
  • Neural progenitor cell
  • Proliferation
  • Sirna
  • Subventricular zone
  • Survival

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology

Cite this

Reproducible expansion and characterization of mouse neural stem/progenitor cells in adherent cultures derived from the adult subventricular zone. / Theus, Michelle H.; Ricard, Jerome; Liebl, Daniel J.

In: Current Protocols in Stem Cell Biology, Vol. 1, No. SUPPL.20, 2D.8, 01.03.2012.

Research output: Contribution to journalArticle

@article{afca995b4f074e30a8ccb830d065f74e,
title = "Reproducible expansion and characterization of mouse neural stem/progenitor cells in adherent cultures derived from the adult subventricular zone",
abstract = "Endogenous neural stem/progenitor cells (NSPCs) residing in the subventricular zone (SVZ) of the adult mouse forebrain have been shown to enhance their neurogenic potential in response to CNS injury. Mechanisms involved in regulating adult neurogenesis under na{\"i}ve or stressed conditions can be studied using a monolayer cell-culture system of the nestin-expressing NSPC lineage to analyze proliferation, survival, and differentiation. Here, a protocol for the expansion of NSPCs for studies aimed at understanding the functional role of NSPCs in maintaining adult neurogenic processes is described. This unit outlines detailed procedures for: (1) isolation, maintenance, and culture of the NSPC component of the SVZ niche from the lateral wall of the lateral ventricle; (2) characterization of NSPC functions by examining proliferation, survival, and differentiation; and (3) efficient siRNA transfection methods in 96-well format.",
keywords = "Cultures, Differentiation, Neural progenitor cell, Proliferation, Sirna, Subventricular zone, Survival",
author = "Theus, {Michelle H.} and Jerome Ricard and Liebl, {Daniel J}",
year = "2012",
month = "3",
day = "1",
doi = "10.1002/9780470151808.sc02d08s20",
language = "English",
volume = "1",
journal = "American Journal of Medical Genetics, Part B: Neuropsychiatric Genetics",
issn = "1552-4841",
publisher = "Wiley-Liss Inc.",
number = "SUPPL.20",

}

TY - JOUR

T1 - Reproducible expansion and characterization of mouse neural stem/progenitor cells in adherent cultures derived from the adult subventricular zone

AU - Theus, Michelle H.

AU - Ricard, Jerome

AU - Liebl, Daniel J

PY - 2012/3/1

Y1 - 2012/3/1

N2 - Endogenous neural stem/progenitor cells (NSPCs) residing in the subventricular zone (SVZ) of the adult mouse forebrain have been shown to enhance their neurogenic potential in response to CNS injury. Mechanisms involved in regulating adult neurogenesis under naïve or stressed conditions can be studied using a monolayer cell-culture system of the nestin-expressing NSPC lineage to analyze proliferation, survival, and differentiation. Here, a protocol for the expansion of NSPCs for studies aimed at understanding the functional role of NSPCs in maintaining adult neurogenic processes is described. This unit outlines detailed procedures for: (1) isolation, maintenance, and culture of the NSPC component of the SVZ niche from the lateral wall of the lateral ventricle; (2) characterization of NSPC functions by examining proliferation, survival, and differentiation; and (3) efficient siRNA transfection methods in 96-well format.

AB - Endogenous neural stem/progenitor cells (NSPCs) residing in the subventricular zone (SVZ) of the adult mouse forebrain have been shown to enhance their neurogenic potential in response to CNS injury. Mechanisms involved in regulating adult neurogenesis under naïve or stressed conditions can be studied using a monolayer cell-culture system of the nestin-expressing NSPC lineage to analyze proliferation, survival, and differentiation. Here, a protocol for the expansion of NSPCs for studies aimed at understanding the functional role of NSPCs in maintaining adult neurogenic processes is described. This unit outlines detailed procedures for: (1) isolation, maintenance, and culture of the NSPC component of the SVZ niche from the lateral wall of the lateral ventricle; (2) characterization of NSPC functions by examining proliferation, survival, and differentiation; and (3) efficient siRNA transfection methods in 96-well format.

KW - Cultures

KW - Differentiation

KW - Neural progenitor cell

KW - Proliferation

KW - Sirna

KW - Subventricular zone

KW - Survival

UR - http://www.scopus.com/inward/record.url?scp=84868144207&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84868144207&partnerID=8YFLogxK

U2 - 10.1002/9780470151808.sc02d08s20

DO - 10.1002/9780470151808.sc02d08s20

M3 - Article

C2 - 22415840

AN - SCOPUS:84868144207

VL - 1

JO - American Journal of Medical Genetics, Part B: Neuropsychiatric Genetics

JF - American Journal of Medical Genetics, Part B: Neuropsychiatric Genetics

SN - 1552-4841

IS - SUPPL.20

M1 - 2D.8

ER -