Reproducible expansion and characterization of mouse neural stem/progenitor cells in adherent cultures derived from the adult subventricular zone

Michelle H. Theus, Jerome Ricard, Daniel J. Liebl

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Endogenous neural stem/progenitor cells (NSPCs) residing in the subventricular zone (SVZ) of the adult mouse forebrain have been shown to enhance their neurogenic potential in response to CNS injury. Mechanisms involved in regulating adult neurogenesis under naïve or stressed conditions can be studied using a monolayer cell-culture system of the nestin-expressing NSPC lineage to analyze proliferation, survival, and differentiation. Here, a protocol for the expansion of NSPCs for studies aimed at understanding the functional role of NSPCs in maintaining adult neurogenic processes is described. This unit outlines detailed procedures for: (1) isolation, maintenance, and culture of the NSPC component of the SVZ niche from the lateral wall of the lateral ventricle; (2) characterization of NSPC functions by examining proliferation, survival, and differentiation; and (3) efficient siRNA transfection methods in 96-well format.

Original languageEnglish (US)
Article number2D.8
JournalCurrent Protocols in Stem Cell Biology
Volume1
Issue numberSUPPL.20
DOIs
StatePublished - Mar 2012

Keywords

  • Cultures
  • Differentiation
  • Neural progenitor cell
  • Proliferation
  • Sirna
  • Subventricular zone
  • Survival

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology

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