Reproducibility of laser Doppler flowmetry in the human optic nerve head

Karen M. Joos, Lutz E. Pillunat, Robert W. Knighton, Douglas R. Anderson, William J. Feuer

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Purpose: We sought to demonstrate reproducibility of the noninvasive laser Doppler flowmetry technique for measuring microvascular hemodynamics of the optic nerve head in human subjects. Methods: Laser Doppler flowmetry was performed by four examiners on four human optic nerve heads during multiple sessions. Intersubject, interexaminer, intersession, and intrasession variabilities were calculated for velocity, blood volume, and blood flow (flux). Results: Initial variability of measurements improved after 3 months' experience. The standard deviation of the intrasession variation for single readings was 18% of the velocity mean value and 24% of the flow (flux) mean value. Based on this, a sample size of 17 (power β = 0.8, a: = 0.05, paired t test) will demonstrate a 10% physiologic change in flux within a session if the average of five readings is used before and after the experimental perturbation. The intersession variation was 12% and 32% for velocity and flux? respectively. Because of this, a larger sample size or additional measurement sessions are required for detection of an experimental influence between two sessions. The coefficient of variation among subjects for single readings was 7% and 10% for velocity and flux, respectively.

Original languageEnglish (US)
Pages (from-to)212-216
Number of pages5
JournalJournal of glaucoma
Issue number4
StatePublished - Aug 1997


  • Blood flow
  • Laser Doppler flowmetry
  • Optic disk
  • Optic nerve head hemodynamics
  • Reproducibility
  • Statistics

ASJC Scopus subject areas

  • Ophthalmology


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