Repression of the chicken c-rel promoter by vRel in chicken embryo fibroblasts is not mediated through a consensus NF-κB binding site

Anthony J Capobianco, Thomas D. Gilmore

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

To understand the regulation of expression of the chicken c-rel gene, we cloned genomic sequences upstream of the start site of transcription of c-rel. Sequence analysis shows that the c-rel promoter is a GC-rich promoter that lacks a TATA box. In addition, there are putative binding sites for several transcription factors, including an NF-κB consensus binding site. Primer extension showed that there is one major start site (site 1) for transcription in chicken embryo fibroblasts and two major start sites in a v-rel-transformed chicken spleen cell line. In transient assays using c-rel promoter sequences and the CAT reporter gene, we found that vRel repressed expression from the c-rel promoter. Other viral oncoproteins and a non-transforming v-rel deletion mutant did not repress the c-rel promoter. Repression occurred through sequences located within 125bp of the start of transcription. However, mutation of the consensus NF-κB binding site did not affect the level of transcription from the c-rel promoter, nor did it interfere with repression by vRel, even though vRel could bind to the wild-type, but not the mutant, version of this sequence in vitro. These results suggest that the vRel protein can repress transcription through an indirect mechanism.

Original languageEnglish
Pages (from-to)2203-2210
Number of pages8
JournalOncogene
Volume6
Issue number12
StatePublished - Dec 1 1991
Externally publishedYes

Fingerprint

Chickens
Embryonic Structures
Fibroblasts
Binding Sites
rel Genes
TATA Box
Transcription Initiation Site
Oncogene Proteins
Reporter Genes
Sequence Analysis
Transcription Factors
Spleen
Cell Line
Mutation
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Repression of the chicken c-rel promoter by vRel in chicken embryo fibroblasts is not mediated through a consensus NF-κB binding site. / Capobianco, Anthony J; Gilmore, Thomas D.

In: Oncogene, Vol. 6, No. 12, 01.12.1991, p. 2203-2210.

Research output: Contribution to journalArticle

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