Repression of small toxic protein synthesis by the Sib and OhsC small RNAs

Elizabeth M. Fozo, Mitsuoki Kawano, Fanette Fontaine, Yusuf Kaya, Kathy S. Mendieta, Kristi L. Jones, Alejandro Ocampo, Kenneth E. Rudd, Gisela Storz

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

The sequences encoding the QUAD1 RNAs were initially identified as four repeats in Escherichia coli. These repeats, herein renamed SIB, are conserved in closely related bacteria, although the number of repeats varies. All five Sib RNAs in E. coli MG1655 are expressed, and no phenotype was observed for a five-sib deletion strain. However, a phenotype reminiscent of plasmid addiction was observed for overexpression of the Sib RNAs, and further examination of the SIB repeat sequences revealed conserved open reading frames encoding highly hydrophobic 18- to 19-amino-acid proteins (Ibs) opposite each sib gene. The Ibs proteins were found to be toxic when overexpressed and this toxicity could be prevented by coexpression of the corresponding Sib RNA. Two other RNAs encoded divergently in the yfhL-acpS intergenic region were similarly found to encode a small hydrophobic protein (ShoB) and an antisense RNA regulator (OhsC). Overexpression of both IbsC and ShoB led to immediate changes in membrane potential suggesting both proteins affect the cell envelope. Whole genome expression analysis showed that overexpression of IbsC and ShoB, as well as the small hydrophobic LdrD and TisB proteins, has both overlapping and unique consequences for the cell.

Original languageEnglish
Pages (from-to)1076-1093
Number of pages18
JournalMolecular Microbiology
Volume70
Issue number5
DOIs
StatePublished - Dec 1 2008
Externally publishedYes

Fingerprint

Poisons
RNA
Proteins
Escherichia coli
Phenotype
Antisense RNA
Intergenic DNA
Conserved Sequence
Membrane Potentials
Open Reading Frames
Plasmids
Genome
Bacteria
Amino Acids
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Fozo, E. M., Kawano, M., Fontaine, F., Kaya, Y., Mendieta, K. S., Jones, K. L., ... Storz, G. (2008). Repression of small toxic protein synthesis by the Sib and OhsC small RNAs. Molecular Microbiology, 70(5), 1076-1093. https://doi.org/10.1111/j.1365-2958.2008.06394.x

Repression of small toxic protein synthesis by the Sib and OhsC small RNAs. / Fozo, Elizabeth M.; Kawano, Mitsuoki; Fontaine, Fanette; Kaya, Yusuf; Mendieta, Kathy S.; Jones, Kristi L.; Ocampo, Alejandro; Rudd, Kenneth E.; Storz, Gisela.

In: Molecular Microbiology, Vol. 70, No. 5, 01.12.2008, p. 1076-1093.

Research output: Contribution to journalArticle

Fozo, EM, Kawano, M, Fontaine, F, Kaya, Y, Mendieta, KS, Jones, KL, Ocampo, A, Rudd, KE & Storz, G 2008, 'Repression of small toxic protein synthesis by the Sib and OhsC small RNAs', Molecular Microbiology, vol. 70, no. 5, pp. 1076-1093. https://doi.org/10.1111/j.1365-2958.2008.06394.x
Fozo EM, Kawano M, Fontaine F, Kaya Y, Mendieta KS, Jones KL et al. Repression of small toxic protein synthesis by the Sib and OhsC small RNAs. Molecular Microbiology. 2008 Dec 1;70(5):1076-1093. https://doi.org/10.1111/j.1365-2958.2008.06394.x
Fozo, Elizabeth M. ; Kawano, Mitsuoki ; Fontaine, Fanette ; Kaya, Yusuf ; Mendieta, Kathy S. ; Jones, Kristi L. ; Ocampo, Alejandro ; Rudd, Kenneth E. ; Storz, Gisela. / Repression of small toxic protein synthesis by the Sib and OhsC small RNAs. In: Molecular Microbiology. 2008 ; Vol. 70, No. 5. pp. 1076-1093.
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