Report on a patient with paroxysmal cold hemoglobinuria

Pochi Ramalingam Subbarayan, Tsutomu Shichishima, Hiroshi Yoshida, Sachio Maruyama, Masaharu Naiki

Research output: Contribution to journalArticlepeer-review


Antibodies against the human blood group P antigen (anti-P alloantibodies) agglutinate phenotype P1 and P2 erythrocytes treated with papain at 4°C but not phenotype P(K) and p erythrocytes. This condition is referred to as an autoimmune disease of paroxysmal cold hemoglobinuria (PCH), and the anti-P specificity is found in the cold hemagglutinins. Serum from a patient suspected to be suffering from PCH by the cold auto-agglutination properties was tested for anti-P specificity, using papain-treated O blood group erythrocytes. The serum-mediated hemagglutination and the serum and complement-mediated immunohemolysis were inhibited by globoside (P antigen GalNAcβ1-3Galα1-4Galβ1-4Glc-ceramide) and Forssman glycosphingolipid (GalNAcα1-3GalNAcβ1-3Galα1-4Galβ1-4Glc-ceramide). Therefore, we concluded that she had PCH. She was completely cured 6 months later.

Original languageEnglish (US)
Pages (from-to)165-167
Number of pages3
JournalInternational journal of hematology
Issue number2
StatePublished - Feb 1997


  • Auto-hemoagglutination
  • Donath and Landsteiner
  • P antigen
  • PCH

ASJC Scopus subject areas

  • Hematology


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