Replicative intermediate of hepatitis B virus in transfected murine fibroblasts

Arthur Z Zelent, M. A. Sells, M. Shvartsman, P. M. Price, G. Acs

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The NIH 3T3-derived cell line ψAM22b, which carries a defective Moloney murine leukemia virus, was transfected with a plasmid carrying the neo gene and two head-to-tail copies of the hepatitis B virus (HBV) genome positioned with opposing polarities. Both the two HBV dimers and the neo gene were located between two Moloney murine leukemia virus long terminal repeats. Poly(A)+ RNAs isolated from one clone that grew in the presence of G418 contained the two major classes of HBV-specific transcripts (3.5-kilobase pregenome and 2.1-kilobase mRNAs) in approximately equivalent amounts, which was reminiscent of the profiles of viral mRNAs from the livers of infected humans and chimpanzees.

Original languageEnglish (US)
Pages (from-to)2921-2923
Number of pages3
JournalJournal of Virology
Volume61
Issue number9
StatePublished - 1987
Externally publishedYes

Fingerprint

Hepatitis B virus
Murine leukemia virus
Moloney murine leukemia virus
fibroblasts
Fibroblasts
Messenger RNA
mice
Defective Viruses
NIH 3T3 Cells
terminal repeat sequences
Pan troglodytes
Terminal Repeat Sequences
messenger RNA
Genes
plasmids
Plasmids
genes
Clone Cells
cell lines
Genome

ASJC Scopus subject areas

  • Immunology

Cite this

Zelent, A. Z., Sells, M. A., Shvartsman, M., Price, P. M., & Acs, G. (1987). Replicative intermediate of hepatitis B virus in transfected murine fibroblasts. Journal of Virology, 61(9), 2921-2923.

Replicative intermediate of hepatitis B virus in transfected murine fibroblasts. / Zelent, Arthur Z; Sells, M. A.; Shvartsman, M.; Price, P. M.; Acs, G.

In: Journal of Virology, Vol. 61, No. 9, 1987, p. 2921-2923.

Research output: Contribution to journalArticle

Zelent, AZ, Sells, MA, Shvartsman, M, Price, PM & Acs, G 1987, 'Replicative intermediate of hepatitis B virus in transfected murine fibroblasts', Journal of Virology, vol. 61, no. 9, pp. 2921-2923.
Zelent AZ, Sells MA, Shvartsman M, Price PM, Acs G. Replicative intermediate of hepatitis B virus in transfected murine fibroblasts. Journal of Virology. 1987;61(9):2921-2923.
Zelent, Arthur Z ; Sells, M. A. ; Shvartsman, M. ; Price, P. M. ; Acs, G. / Replicative intermediate of hepatitis B virus in transfected murine fibroblasts. In: Journal of Virology. 1987 ; Vol. 61, No. 9. pp. 2921-2923.
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