Repair of peripheral nerve defects in rabbits using keratin hydrogel scaffolds

Paulina S. Hill, Peter J. Apel, Jonathan Barnwell, Tom Smith, L. Andrew Koman, Anthony Atala, Mark Van Dyke

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Entubulation of transected nerves using bioabsorbable conduits is a promising alternative to sural nerve autografting, but full functional recovery is rarely achieved. Numerous studies have suggested that scaffold-based conduit fillers may promote axon regeneration, but no neuroinductive biomaterial filler has been identified. We previously showed that a nerve guide filled with keratin hydrogel actively stimulates regeneration in a mouse model, and results in functional outcomes superior to empty conduits at early time points. The goal of the present study was to develop a peripheral nerve defect model in a rabbit and assess the effectiveness of a keratin hydrogel filler. Although repairs with keratin-filled conduits were not as consistently successful as autograft overall, the use of keratin resulted in a significant improvement in conduction delay compared to both empty conduits and autograft, as well as a significant improvement in amplitude recovery compared to empty conduits when measurable regeneration did occur. Taking into account all study animals (i.e., regenerated and nonregenerated), histological assessment showed that keratin-treated nerves had significantly greater myelin thickness than empty conduits. These data support the findings of our earlier study and suggest that keratin hydrogel fillers have the potential to be used clinically to improve conduit repair.

Original languageEnglish
Pages (from-to)1499-1505
Number of pages7
JournalTissue Engineering - Part A
Volume17
Issue number11-12
DOIs
StatePublished - Jun 1 2011
Externally publishedYes

Fingerprint

Keratin
Hydrogel
Scaffolds (biology)
Keratins
Peripheral Nerves
Hydrogels
Scaffolds
Repair
Rabbits
Defects
Fillers
Regeneration
Autografts
Recovery
Sural Nerve
Autologous Transplantation
Biocompatible Materials
Myelin Sheath
Biomaterials
Axons

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

Cite this

Hill, P. S., Apel, P. J., Barnwell, J., Smith, T., Koman, L. A., Atala, A., & Van Dyke, M. (2011). Repair of peripheral nerve defects in rabbits using keratin hydrogel scaffolds. Tissue Engineering - Part A, 17(11-12), 1499-1505. https://doi.org/10.1089/ten.tea.2010.0184

Repair of peripheral nerve defects in rabbits using keratin hydrogel scaffolds. / Hill, Paulina S.; Apel, Peter J.; Barnwell, Jonathan; Smith, Tom; Koman, L. Andrew; Atala, Anthony; Van Dyke, Mark.

In: Tissue Engineering - Part A, Vol. 17, No. 11-12, 01.06.2011, p. 1499-1505.

Research output: Contribution to journalArticle

Hill, PS, Apel, PJ, Barnwell, J, Smith, T, Koman, LA, Atala, A & Van Dyke, M 2011, 'Repair of peripheral nerve defects in rabbits using keratin hydrogel scaffolds', Tissue Engineering - Part A, vol. 17, no. 11-12, pp. 1499-1505. https://doi.org/10.1089/ten.tea.2010.0184
Hill PS, Apel PJ, Barnwell J, Smith T, Koman LA, Atala A et al. Repair of peripheral nerve defects in rabbits using keratin hydrogel scaffolds. Tissue Engineering - Part A. 2011 Jun 1;17(11-12):1499-1505. https://doi.org/10.1089/ten.tea.2010.0184
Hill, Paulina S. ; Apel, Peter J. ; Barnwell, Jonathan ; Smith, Tom ; Koman, L. Andrew ; Atala, Anthony ; Van Dyke, Mark. / Repair of peripheral nerve defects in rabbits using keratin hydrogel scaffolds. In: Tissue Engineering - Part A. 2011 ; Vol. 17, No. 11-12. pp. 1499-1505.
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