Repair of a segmental long bone defect in human by implantation of a novel multiple disc graft

Eric Hesse, Gerald Kluge, Azeddine Atfi, Diego Correa, Carl Haasper, Georg Berding, Hoen oh Shin, Jörg Viering, Florian Länger, Peter M. Vogt, Christian Krettek, Michael Jagodzinski

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


Large segmental defects of the weight bearing long bones are very difficult to reconstruct. Current treatment options are afflicted with several limitations and disadvantages. We describe a novel approach to regenerate a segmental long bone defect in a patient using a multiple disc graft. Decellularized bovine trabecular bone discs were seeded with autologous bone marrow cells and cultured in a perfusion chamber for three weeks. Multiple cell-seeded discs were implanted to close a 72. mm defect of the distal tibia in a 58-year-old woman, and fixed by an intramedullary nail. Bone formation was assessed non-invasively by plain radiographs and 18F-labeled sodium fluoride-based co-registration of positron emission- and computed tomography (PET/CT). Bone was actively formed around the grafted defect as early as six weeks after surgery. Because the tibia was sufficiently stabilized, the patient was able to freely walk with full weight bearing 6. weeks after surgery. The uneventful two-year follow-up and the satisfaction of the patient demonstrated the success of the procedure. Therefore the use of multiple cell-seeded disc grafts can be considered as a treatment alternative for patients with segmental long bone defects.

Original languageEnglish (US)
Pages (from-to)1457-1463
Number of pages7
Issue number5
StatePublished - May 2010
Externally publishedYes


  • 18F-NaF PET/CT
  • Bone graft
  • Bone marrow stromal cell
  • Long bone defect
  • Regenerative medicine

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Histology


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