Reovirus-induced apoptosis of MDCK cells is not linked to viral yield and is blocked by Bcl-2

Steven Rodgers, E. S. Barton, S. M. Oberhaus, B. Pike, C. A. Gibson, K. L. Tyler, T. S. Dermody

Research output: Contribution to journalArticle

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Abstract

In this study, we investigated the relationship between reovirus- induced apoptosis and viral growth. Madin-Darby canine kidney (MDCK) epithelial cells infected with prototype reovirus strains type 1 Lang (T1L) or type 3 Dearing (T3D) were found to undergo apoptosis, and T3D induced apoptosis of MDCK cells to a substantially greater extent than T1L. By using T1L x T3D reassortant viruses, we found that differences in the capacities of these strains to induce apoptosis are determined by the viral S1 and M2 gene segments. These genes encode viral outer-capsid proteins that play important roles in viral entry into cells. T1L grew significantly better in MDCK cells than T3D, and these differences in growth segregated with the vital L1 and M1 gene segments. The L1 and M1 genes encode viral core proteins involved in vital RNA synthesis. Bcl-2 nverexpression in MDCK cells inhibited reovirus- induced apoptosis but did not substantially affect reovirus growth. These findings indicate that differences in the capacities of reovirus strains to induce apoptosis and grow in MDCK cells are determined by different vital genes and that premature cell death by apoptosis does not limit reovirus growth in MDCK cells.

Original languageEnglish
Pages (from-to)2540-2546
Number of pages7
JournalJournal of Virology
Volume71
Issue number3
StatePublished - Feb 25 1997
Externally publishedYes

Fingerprint

Reoviridae
Madin Darby Canine Kidney Cells
kidney cells
apoptosis
Apoptosis
dogs
Growth
genes
Genes
Mammalian Orthoreovirus
Reassortant Viruses
Viral Core Proteins
Premature Mortality
Viral Genes
Capsid Proteins
coat proteins
prototypes
microbial growth
cell death
epithelial cells

ASJC Scopus subject areas

  • Immunology

Cite this

Rodgers, S., Barton, E. S., Oberhaus, S. M., Pike, B., Gibson, C. A., Tyler, K. L., & Dermody, T. S. (1997). Reovirus-induced apoptosis of MDCK cells is not linked to viral yield and is blocked by Bcl-2. Journal of Virology, 71(3), 2540-2546.

Reovirus-induced apoptosis of MDCK cells is not linked to viral yield and is blocked by Bcl-2. / Rodgers, Steven; Barton, E. S.; Oberhaus, S. M.; Pike, B.; Gibson, C. A.; Tyler, K. L.; Dermody, T. S.

In: Journal of Virology, Vol. 71, No. 3, 25.02.1997, p. 2540-2546.

Research output: Contribution to journalArticle

Rodgers, S, Barton, ES, Oberhaus, SM, Pike, B, Gibson, CA, Tyler, KL & Dermody, TS 1997, 'Reovirus-induced apoptosis of MDCK cells is not linked to viral yield and is blocked by Bcl-2', Journal of Virology, vol. 71, no. 3, pp. 2540-2546.
Rodgers S, Barton ES, Oberhaus SM, Pike B, Gibson CA, Tyler KL et al. Reovirus-induced apoptosis of MDCK cells is not linked to viral yield and is blocked by Bcl-2. Journal of Virology. 1997 Feb 25;71(3):2540-2546.
Rodgers, Steven ; Barton, E. S. ; Oberhaus, S. M. ; Pike, B. ; Gibson, C. A. ; Tyler, K. L. ; Dermody, T. S. / Reovirus-induced apoptosis of MDCK cells is not linked to viral yield and is blocked by Bcl-2. In: Journal of Virology. 1997 ; Vol. 71, No. 3. pp. 2540-2546.
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