Reovirus σNS protein is required for nucleation of viral assembly complexes and formation of viral inclusions

M. M. Becker, M. I. Goral, P. R. Hazelton, G. S. Baer, Steven Rodgers, E. G. Brown, K. M. Coombs, T. S. Dermody

Research output: Contribution to journalArticle

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Abstract

Progeny virions of mammalian reoviruses are assembled in the cytoplasm of infected cells at discrete sites termed viral inclusions. Studies of temperature-sensitive (ts) mutant viruses indicate that nonstructural protein σNS and core protein μ2 are required for synthesis of double-stranded (ds) RNA, a process that occurs at sites of viral assembly. We used confocal immunofluorescence microscopy and ts mutant reoviruses to define the roles of σNS and μ2 in viral inclusion formation. In cells infected with wild-type (wt) reovirus, σNS and μ2 colocalize to large, perinuclear structures that correspond to viral inclusions. In cells infected at a nonpermissive temperature with σNS-mutant virus tsE320, σNS is distributed diffusely in the cytoplasm and μ2 is contained in small, punctate foci that do not resemble viral inclusions. In cells infected at a nonpermissive temperature with μ2-mutant virus tsH11.2, μ2 is distributed diffusely in the cytoplasm and the nucleus. However, σNS localizes to discrete structures in the cytoplasm that contain other viral proteins and are morphologically indistinguishable from viral inclusions seen in cells infected with wt reovirus. Examination of cells infected with wt reovirus over a time course demonstrates that σNS precedes μ2 in localization to viral inclusions. These findings suggest that viral RNA-protein complexes containing σNS nucleate sites of viral replication to which other viral proteins, including μ2, are recruited to commence dsRNA synthesis.

Original languageEnglish
Pages (from-to)1459-1475
Number of pages17
JournalJournal of Virology
Volume75
Issue number3
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

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Virus Assembly
Reoviridae
Cytoplasm
Viral Proteins
cytoplasm
mutants
Proteins
Temperature
proteins
viral proteins
double-stranded RNA
cells
Viruses
viruses
temperature
Mammalian Orthoreovirus
synthesis
Double-Stranded RNA
Viral RNA
virus replication

ASJC Scopus subject areas

  • Immunology

Cite this

Reovirus σNS protein is required for nucleation of viral assembly complexes and formation of viral inclusions. / Becker, M. M.; Goral, M. I.; Hazelton, P. R.; Baer, G. S.; Rodgers, Steven; Brown, E. G.; Coombs, K. M.; Dermody, T. S.

In: Journal of Virology, Vol. 75, No. 3, 01.01.2001, p. 1459-1475.

Research output: Contribution to journalArticle

Becker, MM, Goral, MI, Hazelton, PR, Baer, GS, Rodgers, S, Brown, EG, Coombs, KM & Dermody, TS 2001, 'Reovirus σNS protein is required for nucleation of viral assembly complexes and formation of viral inclusions', Journal of Virology, vol. 75, no. 3, pp. 1459-1475. https://doi.org/10.1128/JVI.75.3.1459-1475.2001
Becker, M. M. ; Goral, M. I. ; Hazelton, P. R. ; Baer, G. S. ; Rodgers, Steven ; Brown, E. G. ; Coombs, K. M. ; Dermody, T. S. / Reovirus σNS protein is required for nucleation of viral assembly complexes and formation of viral inclusions. In: Journal of Virology. 2001 ; Vol. 75, No. 3. pp. 1459-1475.
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