Renal plasticity in response to feeding in the Burmese python, Python molurus bivittatus

A. J. Esbaugh, S. M. Secor, Martin Grosell

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Burmese pythons are sit-and-wait predators that are well adapted to go long periods without food, yet subsequently consume and digest single meals that can exceed their body weight. These large feeding events result in a dramatic alkaline tide that is compensated by a hypoventilatory response that normalizes plasma pH; however, little is known regarding how plasma HCO<inf>3</inf><sup>-</sup> is lowered in the days post-feeding. The current study demonstrated that Burmese pythons contain the cellular machinery for renal acid-base compensation and actively remodel the kidney to limit HCO<inf>3</inf><sup>-</sup> reabsorption in the post-feeding period. After being fed a 25% body weight meal plasma total CO<inf>2</inf> was elevated by 1.5-fold after 1day, but returned to control concentrations by 4days post-feeding (dpf). Gene expression analysis was used to verify the presence of carbonic anhydrase (CA) II, IV and XIII, Na<sup>+</sup> H<sup>+</sup> exchanger 3 (NHE3), the Na<sup>+</sup> HCO<inf>3</inf><sup>-</sup> co-transporter (NBC) and V-type ATPase. CA IV expression was significantly down-regulated at 3dpf versus fasted controls. This was supported by activity analysis that showed a significant decrease in the amount of GPI-linked CA activity in isolated kidney membranes at 3dpf versus fasted controls. In addition, V-type ATPase activity was significantly up-regulated at 3dpf; no change in gene expression was observed. Both CA II and NHE3 expression was up-regulated at 3dpf, which may be related to post-prandial ion balance. These results suggest that Burmese pythons actively remodel their kidney after feeding, which would in part benefit renal HCO<inf>3</inf><sup>-</sup> clearance.

Original languageEnglish (US)
Pages (from-to)120-126
Number of pages7
JournalComparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
Volume188
DOIs
StatePublished - Oct 1 2015

Fingerprint

Boidae
Plasticity
Carbonic Anhydrase IV
Kidney
Carbonic Anhydrase II
Vacuolar Proton-Translocating ATPases
Meals
Sodium-Hydrogen Antiporter
Plasmas
Gene expression
Body Weight
Symporters
Gene Expression
Carbonic Anhydrases
Tides
Machinery
Ions
Membranes
Food
Acids

Keywords

  • Acid-base balance
  • Alkaline tide
  • CA IV
  • Carbonic anhydrase
  • Kidney
  • Specific dynamic action
  • V-type ATPase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Physiology

Cite this

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title = "Renal plasticity in response to feeding in the Burmese python, Python molurus bivittatus",
abstract = "Burmese pythons are sit-and-wait predators that are well adapted to go long periods without food, yet subsequently consume and digest single meals that can exceed their body weight. These large feeding events result in a dramatic alkaline tide that is compensated by a hypoventilatory response that normalizes plasma pH; however, little is known regarding how plasma HCO3- is lowered in the days post-feeding. The current study demonstrated that Burmese pythons contain the cellular machinery for renal acid-base compensation and actively remodel the kidney to limit HCO3- reabsorption in the post-feeding period. After being fed a 25{\%} body weight meal plasma total CO2 was elevated by 1.5-fold after 1day, but returned to control concentrations by 4days post-feeding (dpf). Gene expression analysis was used to verify the presence of carbonic anhydrase (CA) II, IV and XIII, Na+ H+ exchanger 3 (NHE3), the Na+ HCO3- co-transporter (NBC) and V-type ATPase. CA IV expression was significantly down-regulated at 3dpf versus fasted controls. This was supported by activity analysis that showed a significant decrease in the amount of GPI-linked CA activity in isolated kidney membranes at 3dpf versus fasted controls. In addition, V-type ATPase activity was significantly up-regulated at 3dpf; no change in gene expression was observed. Both CA II and NHE3 expression was up-regulated at 3dpf, which may be related to post-prandial ion balance. These results suggest that Burmese pythons actively remodel their kidney after feeding, which would in part benefit renal HCO3- clearance.",
keywords = "Acid-base balance, Alkaline tide, CA IV, Carbonic anhydrase, Kidney, Specific dynamic action, V-type ATPase",
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T1 - Renal plasticity in response to feeding in the Burmese python, Python molurus bivittatus

AU - Esbaugh, A. J.

AU - Secor, S. M.

AU - Grosell, Martin

PY - 2015/10/1

Y1 - 2015/10/1

N2 - Burmese pythons are sit-and-wait predators that are well adapted to go long periods without food, yet subsequently consume and digest single meals that can exceed their body weight. These large feeding events result in a dramatic alkaline tide that is compensated by a hypoventilatory response that normalizes plasma pH; however, little is known regarding how plasma HCO3- is lowered in the days post-feeding. The current study demonstrated that Burmese pythons contain the cellular machinery for renal acid-base compensation and actively remodel the kidney to limit HCO3- reabsorption in the post-feeding period. After being fed a 25% body weight meal plasma total CO2 was elevated by 1.5-fold after 1day, but returned to control concentrations by 4days post-feeding (dpf). Gene expression analysis was used to verify the presence of carbonic anhydrase (CA) II, IV and XIII, Na+ H+ exchanger 3 (NHE3), the Na+ HCO3- co-transporter (NBC) and V-type ATPase. CA IV expression was significantly down-regulated at 3dpf versus fasted controls. This was supported by activity analysis that showed a significant decrease in the amount of GPI-linked CA activity in isolated kidney membranes at 3dpf versus fasted controls. In addition, V-type ATPase activity was significantly up-regulated at 3dpf; no change in gene expression was observed. Both CA II and NHE3 expression was up-regulated at 3dpf, which may be related to post-prandial ion balance. These results suggest that Burmese pythons actively remodel their kidney after feeding, which would in part benefit renal HCO3- clearance.

AB - Burmese pythons are sit-and-wait predators that are well adapted to go long periods without food, yet subsequently consume and digest single meals that can exceed their body weight. These large feeding events result in a dramatic alkaline tide that is compensated by a hypoventilatory response that normalizes plasma pH; however, little is known regarding how plasma HCO3- is lowered in the days post-feeding. The current study demonstrated that Burmese pythons contain the cellular machinery for renal acid-base compensation and actively remodel the kidney to limit HCO3- reabsorption in the post-feeding period. After being fed a 25% body weight meal plasma total CO2 was elevated by 1.5-fold after 1day, but returned to control concentrations by 4days post-feeding (dpf). Gene expression analysis was used to verify the presence of carbonic anhydrase (CA) II, IV and XIII, Na+ H+ exchanger 3 (NHE3), the Na+ HCO3- co-transporter (NBC) and V-type ATPase. CA IV expression was significantly down-regulated at 3dpf versus fasted controls. This was supported by activity analysis that showed a significant decrease in the amount of GPI-linked CA activity in isolated kidney membranes at 3dpf versus fasted controls. In addition, V-type ATPase activity was significantly up-regulated at 3dpf; no change in gene expression was observed. Both CA II and NHE3 expression was up-regulated at 3dpf, which may be related to post-prandial ion balance. These results suggest that Burmese pythons actively remodel their kidney after feeding, which would in part benefit renal HCO3- clearance.

KW - Acid-base balance

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KW - Kidney

KW - Specific dynamic action

KW - V-type ATPase

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JO - Comparative Biochemistry & Physiology; A: Comparative Physiology

JF - Comparative Biochemistry & Physiology; A: Comparative Physiology

SN - 1095-6433

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