Renal function in the freshwater rainbow trout (Oncorhynchus mykiss) following acute and prolonged exposure to waterborne nickel

Eric F. Pane, Carol Bucking, Monika Patel, Chris M. Wood

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Renal function was investigated in adult rainbow trout following acute and prolonged exposure to waterborne Ni in moderately hard Lake Ontario water (∼140 mg L-1 as CaCO3). Fish were exposed for 36 days to a sublethal concentration of 442 μg Ni L-1, followed by 96 h of exposure to 12,850 μg Ni L-1 (approximately 33% of the 96 h LC50). Prolonged exposure markedly affected only the renal handling of Ni, with no substantial effect on the plasma concentration, urinary excretion rate (UER) or clearance ratio (CR) of Na+, Cl-, K+, Ca2+, Mg2+, inorganic phosphate (Pi), glucose, lactate, total ammonia (Tamm), protein and free amino acids (FAA). Glomerular filtration rate (GFR) was reduced by 75% over 96 h of acute Ni challenge in both fish previously exposed to Ni and naïve fish, with no significant change in urine flow rate (UFR), suggesting a substantial reduction in water reabsorption to maintain urine flow and water balance. Renal Mg 2+ handling was specifically impaired by acute Ni challenge, leading to a significantly increased UERMg2+ and significantly decreased plasma [Mg 2+] only in naïve fish. Previously-exposed fish were well-protected against Ni-induced Mg2+ antagonism, indicating true acclimation to Ni. Only in naïve, acutely challenged fish was there an increased UER of titratable acidity (TA-HCO3), net acidic equivalents, Pi, Tamm and K+. Again, all of these parameters were well-conserved in previously-exposed fish during acute Ni exposure, strongly suggesting that prolonged, sublethal exposure protected against acute Ni-induced respiratory toxicity.

Original languageEnglish (US)
Pages (from-to)119-133
Number of pages15
JournalAquatic Toxicology
Volume72
Issue number1-2 SPEC. ISS.
DOIs
StatePublished - Mar 25 2005

Keywords

  • Acclimation
  • Nickel
  • Prolonged exposure
  • Rainbow trout
  • Renal physiology

ASJC Scopus subject areas

  • Aquatic Science

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