Removal of N-Linked Glycosylation Sites in the V1 Region of Simian Immunodeficiency Virus gp120 Results in Redirection of B-Cell Responses to V3

Kelly Stefano Cole, Jonathan D. Steckbeck, Jennifer L. Rowles, Ronald Charles Desrosiers, Ronald C. Montelaro

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

One mechanism of immune evasion utilized by human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) envelope glycoproteins is the presence of a dense carbohydrate shield. Accumulating evidence from in vitro and in vivo experiments suggests that alterations in N-linked glycosylation of SIV gp120 can enhance host humoral immune responses that may be involved in immune control. The present study was designed to determine the ability of glycosylation mutant viruses to redirect antibody responses to shielded envelope epitopes. The influence of glycosylation on the maturation and specificity of antibody responses elicited by glycosylation mutant viruses containing mutations of specific N-linked sites in and near the V1 and V2 regions of SIVmac239 gp120 was determined. Results from these studies demonstrated a remarkably similar maturation of antibody responses to native, fully glycosylated envelope proteins. However, analyses of antibodies to defined envelope domains revealed that mutation of glycosylation sites in V1 resulted in increased antibody recognition to epitopes in VI. In addition, we demonstrated for the first time that mutation of glycosylation sites in V1 resulted in a redirection of antibody responses to the V3 loop. Taken together, these results demonstrate that N-linked glycosylation is a determinant of SIV envelope B-cell immunogenicity in addition to in vitro antigenicity. In addition, our results demonstrate that the absence of N-linked carbohydrates at specific sites can influence the exposure of epitopes quite distant in the linear sequence.

Original languageEnglish (US)
Pages (from-to)1525-1539
Number of pages15
JournalJournal of Virology
Volume78
Issue number3
DOIs
StatePublished - Feb 2004
Externally publishedYes

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Simian immunodeficiency virus
glycosylation
Glycosylation
B-lymphocytes
B-Lymphocytes
Antibody Formation
antibodies
epitopes
Epitopes
Simian Immunodeficiency Virus
mutation
Mutation
Carbohydrates
immune evasion
Viruses
carbohydrates
Immune Evasion
viruses
mutants
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Removal of N-Linked Glycosylation Sites in the V1 Region of Simian Immunodeficiency Virus gp120 Results in Redirection of B-Cell Responses to V3. / Cole, Kelly Stefano; Steckbeck, Jonathan D.; Rowles, Jennifer L.; Desrosiers, Ronald Charles; Montelaro, Ronald C.

In: Journal of Virology, Vol. 78, No. 3, 02.2004, p. 1525-1539.

Research output: Contribution to journalArticle

Cole, Kelly Stefano ; Steckbeck, Jonathan D. ; Rowles, Jennifer L. ; Desrosiers, Ronald Charles ; Montelaro, Ronald C. / Removal of N-Linked Glycosylation Sites in the V1 Region of Simian Immunodeficiency Virus gp120 Results in Redirection of B-Cell Responses to V3. In: Journal of Virology. 2004 ; Vol. 78, No. 3. pp. 1525-1539.
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