Remote ischaemic preconditioning and prevention of cerebral injury

Ashish K. Rehni, Richa Shri, Manjeet Singh

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Bilateral carotid artery occlusion of 10 min followed by reperfusion for 24 hr was employed in present study to produce ischaemia and reperfusion induced cerebral injury in mice. Cerebral infarct size was measured using triphenyltetrazolium chloride staining. Short-term memory was evaluated using elevated plus maze. Inclined beam walking test was employed to assess motor incoordination. Bilateral carotid artery occlusion followed by reperfusion produced cerebral infarction and impaired short-term memory, motor co-ordination and lateral push response. A preceding episode of mesenteric artery occlusion for 15 min and reperfusion of 15 min (remote mesenteric ischaemic preconditioning) prevented markedly ischaemia-reperfusion-induced cerebral injury measured in terms of infarct size, loss of short-term memory, motor coordination and lateral push response. Glibenclamide (5 mg/kg, iv) a KATP channel blocker and caffeine (7 mg/kg, iv) an adenosine receptor blocker attenuated the neuroprotective effect of remote mesenteric ischaemic preconditioning. It may be concluded that neuroprotective effect of remote mesenteric ischaemic preconditioning may be due to activation of adenosine receptors and consequent activation of KATP channels in mice.

Original languageEnglish (US)
Pages (from-to)247-252
Number of pages6
JournalIndian Journal of Experimental Biology
Volume45
Issue number3
StatePublished - Mar 2007
Externally publishedYes

Keywords

  • Cerebral injury
  • Ischaemic preconditioning
  • Reperfusion

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Remote ischaemic preconditioning and prevention of cerebral injury'. Together they form a unique fingerprint.

Cite this