Relevance of dopamine signals anchoring dynamin-2 to the plasma membrane during Na+,K+-ATPase endocytosis

Riad Efendiev, Guillermo A. Yudowski, Jean Zwiller, Barbara Leibiger, Adrian I. Katz, Per Olof Berggren, Carlos H. Pedemonte, Ingo B. Leibiger, Alejandro M. Bertorello

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Clathrin-dependent endocytosis of Na+,K+-ATPase in response to dopamine regulates its catalytic activity in intact cells. Because fission of clathrin-coated pits requires dynamin, we examined the mechanisms by which dopamine receptor signals promote dynamin-2 recruitment and assembly at the site of Na+,K+-ATPase endocytosis. Western blotting revealed that dopamine increased the association of dynamin-2 with the plasma membrane and with phosphatidylinositol 3-kinase. Dopamine inhibited Na+,K+-ATPase activity in OK cells and in those overexpressing wild type dynamin-2 but not in cells expressing a dominant-negative mutant. Dephosphorylation of dynamin is important for its assembly. Dopamine increased protein phosphatase 2A activity and dephosphorylated dynamin-2. In cells expressing a dominant-negative mutant of protein phosphatase 2A, dopamine failed to dephosphorylate dynamin-2 and to reduce Na+,K+-ATPase activity. Dynamin-2 is phosphorylated at Ser848, and expression of the S848A mutant significantly blocked the inhibitory effect of dopamine. These results demonstrate a distinct signaling network originating from the dopamine receptor that regulates the state of dynamin-2 phosphorylation and that promotes its location (by interaction with phosphatidylinositol 3-kinase) at the site of Na+,K+-ATPase endocytosis.

Original languageEnglish
Pages (from-to)44108-44114
Number of pages7
JournalJournal of Biological Chemistry
Volume277
Issue number46
DOIs
StatePublished - Nov 15 2002
Externally publishedYes

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Dynamin II
Cell membranes
Endocytosis
Adenosine Triphosphatases
Dopamine
Cell Membrane
Phosphatidylinositol 3-Kinase
Dynamins
Protein Phosphatase 2
Clathrin
Dopamine Receptors
Cells
Phosphorylation
Mutant Proteins
sodium-translocating ATPase
Catalyst activity
Western Blotting
Association reactions

ASJC Scopus subject areas

  • Biochemistry

Cite this

Efendiev, R., Yudowski, G. A., Zwiller, J., Leibiger, B., Katz, A. I., Berggren, P. O., ... Bertorello, A. M. (2002). Relevance of dopamine signals anchoring dynamin-2 to the plasma membrane during Na+,K+-ATPase endocytosis. Journal of Biological Chemistry, 277(46), 44108-44114. https://doi.org/10.1074/jbc.M205173200

Relevance of dopamine signals anchoring dynamin-2 to the plasma membrane during Na+,K+-ATPase endocytosis. / Efendiev, Riad; Yudowski, Guillermo A.; Zwiller, Jean; Leibiger, Barbara; Katz, Adrian I.; Berggren, Per Olof; Pedemonte, Carlos H.; Leibiger, Ingo B.; Bertorello, Alejandro M.

In: Journal of Biological Chemistry, Vol. 277, No. 46, 15.11.2002, p. 44108-44114.

Research output: Contribution to journalArticle

Efendiev, R, Yudowski, GA, Zwiller, J, Leibiger, B, Katz, AI, Berggren, PO, Pedemonte, CH, Leibiger, IB & Bertorello, AM 2002, 'Relevance of dopamine signals anchoring dynamin-2 to the plasma membrane during Na+,K+-ATPase endocytosis', Journal of Biological Chemistry, vol. 277, no. 46, pp. 44108-44114. https://doi.org/10.1074/jbc.M205173200
Efendiev, Riad ; Yudowski, Guillermo A. ; Zwiller, Jean ; Leibiger, Barbara ; Katz, Adrian I. ; Berggren, Per Olof ; Pedemonte, Carlos H. ; Leibiger, Ingo B. ; Bertorello, Alejandro M. / Relevance of dopamine signals anchoring dynamin-2 to the plasma membrane during Na+,K+-ATPase endocytosis. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 46. pp. 44108-44114.
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