Relationship of multidrug resistance to rhodamine-123 selectivity between carcinoma and normal epithelial cells: Taxol and vinblastine modulate drug efflux

Danièle Brouty-Boyé, Despina Kolonias, Chun Jing Wu, Niramol Savaraj, Theodore Lampidis

Research output: Contribution to journalArticle

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Abstract

Preferential retention and cytotoxicity of Rhodamine-123 (Rho-123) was originally reported in a number of carcinoma cell types isolated from a variety of tissues as compared to normal epithelial cells from a limited number of other tissues. In the present study, we have examined Rho-123 selectivity in normal and tumor cell lines isolated from the same tissue source, i.e., human beast. We found that: (a) in matched pairs of normal and carcinoma breast cells, Rho-123 displays no preferential retention in either cell type; (b) there is no preferential toxicity in carcinoma as compared to normal breast cells; in fact, one of the carcinoma cell lines (MDA-MB231) shows moderate resistance to this dye; (c) all of the human breast cell lines do not express P-glycoprotein-mediated multidrug resistance; (d) the normal monkey kidney epithelial cell line CV-1, which was originally used as a model to demonstrate the relative resistance of normal epithelial cells to this drug, is found to express high levels of the mdr-1 gene, is resistant to other multidrug-resistant drugs (taxol and vinblastine), and its resistance to Rho-123 as well as decreased Rho-123 retention can be reversed by verapamil; and (e) taxol and vinblastine are found to black increased Rho- 123 efflux in CV-1 cells. Thus, overall the data suggest that preferential retention and cytotoxicity of Rho-123 in carcinoma versus normal epithelial cells is related to the differential expression of the mdr-1 gene.

Original languageEnglish
Pages (from-to)1633-1638
Number of pages6
JournalCancer Research
Volume55
Issue number8
StatePublished - Apr 15 1995

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Rhodamine 123
Vinblastine
Multiple Drug Resistance
Paclitaxel
Epithelial Cells
Carcinoma
Pharmaceutical Preparations
Cell Line
Breast
P-Glycoprotein
Verapamil
Tumor Cell Line
Genes
Haplorhini
Coloring Agents
Cell Count
Breast Neoplasms
Kidney

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Relationship of multidrug resistance to rhodamine-123 selectivity between carcinoma and normal epithelial cells : Taxol and vinblastine modulate drug efflux. / Brouty-Boyé, Danièle; Kolonias, Despina; Wu, Chun Jing; Savaraj, Niramol; Lampidis, Theodore.

In: Cancer Research, Vol. 55, No. 8, 15.04.1995, p. 1633-1638.

Research output: Contribution to journalArticle

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