Relationship between severity, necrosis, and apoptosis in five models of experimental acute pancreatitis

A. M. Kaiser, Ashok Saluja, A. Sengupta, M. Saluja, M. L. Steer

Research output: Contribution to journalArticle

288 Citations (Scopus)

Abstract

In an effort to elucidate factors that determine the severity of an attack of acute pancreatitis, we have quantitated the extent of necrosis and of apoptosis in five different models of experimental acute pancreatitis. Severe pancreatitis was induced by obstructing the opossum common bile-pancreatic duct, by administering to mice 12 hourly injections of a supramaximally stimulating dose of caerulein, and by feeding young female mice a choline- deficient, ethionine-supplemented diet. In each of these models of severe pancreatitis, marked necrosis but very little apoptosis was found. Mild pancreatitis was induced by obstructing the rat common bile-pancreatic duct and by infusing rats with a supramaximally stimulating dose of caerulein. In contrast to our findings in severe pancreatitis, mild pancreatitis was characterized by very little necrosis but a high degree of apoptosis. Our finding that the severity of acute pancreatitis is inversely related to the degree of apoptosis suggests that apoptosis may be a teleologically beneficial response to acinar cell injury in general and especially in acute pancreatitis.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume269
Issue number5 38-5
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Pancreatitis
Theoretical Models
Necrosis
Apoptosis
Ceruletide
Pancreatic Ducts
Common Bile Duct
Ethionine
Opossums
Acinar Cells
Choline
Diet
Injections
Wounds and Injuries

Keywords

  • acute disease
  • animal
  • cell death
  • pancreas
  • quantitation

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Relationship between severity, necrosis, and apoptosis in five models of experimental acute pancreatitis. / Kaiser, A. M.; Saluja, Ashok; Sengupta, A.; Saluja, M.; Steer, M. L.

In: American Journal of Physiology - Cell Physiology, Vol. 269, No. 5 38-5, 01.01.1995.

Research output: Contribution to journalArticle

@article{c2ae014914a84a639928c535714efbe2,
title = "Relationship between severity, necrosis, and apoptosis in five models of experimental acute pancreatitis",
abstract = "In an effort to elucidate factors that determine the severity of an attack of acute pancreatitis, we have quantitated the extent of necrosis and of apoptosis in five different models of experimental acute pancreatitis. Severe pancreatitis was induced by obstructing the opossum common bile-pancreatic duct, by administering to mice 12 hourly injections of a supramaximally stimulating dose of caerulein, and by feeding young female mice a choline- deficient, ethionine-supplemented diet. In each of these models of severe pancreatitis, marked necrosis but very little apoptosis was found. Mild pancreatitis was induced by obstructing the rat common bile-pancreatic duct and by infusing rats with a supramaximally stimulating dose of caerulein. In contrast to our findings in severe pancreatitis, mild pancreatitis was characterized by very little necrosis but a high degree of apoptosis. Our finding that the severity of acute pancreatitis is inversely related to the degree of apoptosis suggests that apoptosis may be a teleologically beneficial response to acinar cell injury in general and especially in acute pancreatitis.",
keywords = "acute disease, animal, cell death, pancreas, quantitation",
author = "Kaiser, {A. M.} and Ashok Saluja and A. Sengupta and M. Saluja and Steer, {M. L.}",
year = "1995",
month = "1",
day = "1",
language = "English (US)",
volume = "269",
journal = "American Journal of Physiology - Cell Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "5 38-5",

}

TY - JOUR

T1 - Relationship between severity, necrosis, and apoptosis in five models of experimental acute pancreatitis

AU - Kaiser, A. M.

AU - Saluja, Ashok

AU - Sengupta, A.

AU - Saluja, M.

AU - Steer, M. L.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - In an effort to elucidate factors that determine the severity of an attack of acute pancreatitis, we have quantitated the extent of necrosis and of apoptosis in five different models of experimental acute pancreatitis. Severe pancreatitis was induced by obstructing the opossum common bile-pancreatic duct, by administering to mice 12 hourly injections of a supramaximally stimulating dose of caerulein, and by feeding young female mice a choline- deficient, ethionine-supplemented diet. In each of these models of severe pancreatitis, marked necrosis but very little apoptosis was found. Mild pancreatitis was induced by obstructing the rat common bile-pancreatic duct and by infusing rats with a supramaximally stimulating dose of caerulein. In contrast to our findings in severe pancreatitis, mild pancreatitis was characterized by very little necrosis but a high degree of apoptosis. Our finding that the severity of acute pancreatitis is inversely related to the degree of apoptosis suggests that apoptosis may be a teleologically beneficial response to acinar cell injury in general and especially in acute pancreatitis.

AB - In an effort to elucidate factors that determine the severity of an attack of acute pancreatitis, we have quantitated the extent of necrosis and of apoptosis in five different models of experimental acute pancreatitis. Severe pancreatitis was induced by obstructing the opossum common bile-pancreatic duct, by administering to mice 12 hourly injections of a supramaximally stimulating dose of caerulein, and by feeding young female mice a choline- deficient, ethionine-supplemented diet. In each of these models of severe pancreatitis, marked necrosis but very little apoptosis was found. Mild pancreatitis was induced by obstructing the rat common bile-pancreatic duct and by infusing rats with a supramaximally stimulating dose of caerulein. In contrast to our findings in severe pancreatitis, mild pancreatitis was characterized by very little necrosis but a high degree of apoptosis. Our finding that the severity of acute pancreatitis is inversely related to the degree of apoptosis suggests that apoptosis may be a teleologically beneficial response to acinar cell injury in general and especially in acute pancreatitis.

KW - acute disease

KW - animal

KW - cell death

KW - pancreas

KW - quantitation

UR - http://www.scopus.com/inward/record.url?scp=0028783295&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028783295&partnerID=8YFLogxK

M3 - Article

VL - 269

JO - American Journal of Physiology - Cell Physiology

JF - American Journal of Physiology - Cell Physiology

SN - 0363-6143

IS - 5 38-5

ER -