Regulation of the estrogen receptor in MCF-7 cells by estradiol

Miguel Saceda, Marc E. Lippman, Pierre Chambon, Ralph L. Lindsey, Mathurose Ponglikitmongkol, Montserrat Puente, Mary Beth Martin

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308 Scopus citations

Abstract

The role of estradiol in the regulation of its cognate receptor in MCF-7 cells was investigated in this study. After treatment with 10-9 M estradiol, the level of receptor protein was measured using an enzymeimmunoassay. By 6 h, the receptor protein declined by about 60% from a level of approximately 3.6 to 1.2 fmol/μg DNA. The level of receptor remained suppressed for 24-48 h. Similar results were obtained with an estrogen receptor (ER) binding assay. The steady state level of ER mRNA was determined by an RNase protection assay. Estrogen treatment resulted in a maximum suppression of mRNA by 6 h. Receptor mRNA remained depressed for 48 h. Transcription run on experiments demonstrated a transient decrease of about 90% in ER transcription after 1 h. By 3-6 h transcription increased approximately 2-fold and remained elevated for at least 48 h. These data suggest that estrogen down-regulates ER mRNA by inhibition of ER gene transcription at early times and by a post-transcriptional effect on receptor mRNA at later times.

Original languageEnglish (US)
Pages (from-to)1157-1162
Number of pages6
JournalMolecular Endocrinology
Volume2
Issue number12
DOIs
StatePublished - Dec 1988

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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