Regulation of the estrogen receptor in MCF-7 cells by estradiol

M. Saceda, Marc E Lippman, P. Chambon, R. L. Lindsey, M. Ponglikitmongkol, M. Puente, M. B. Martin

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Abstract

The role of estradiol in the regulation of its cognate receptor in MCF-7 cells was investigated in this study. After treatment with 10-9 M estradiol, the level of receptor protein was measured using an enzymeimmunoassay. By 6 h, the receptor protein declined by about 60% from a level of approximately 3.6 to 1.2 fmol/μg DNA. The level of receptor remained suppressed for 24-48 h. Similar results were obtained with an estrogen receptor (ER) binding assay. The steady state level of ER mRNA was determined by an RNase protection assay. Estrogen treatment resulted in a maximum suppression of mRNA by 6 h. Receptor mRNA remained depressed for 48 h. Transcription run on experiments demonstrated a transient decrease of about 90% in ER transcription after 1 h. By 3-6 h transcription increased approximately 2-fold and remained elevated for at least 48 h. These data suggest that estrogen down-regulates ER mRNA by inhibition of ER gene transcription at early times and by a post-transcriptional effect on receptor mRNA at later times.

Original languageEnglish
Pages (from-to)1157-1162
Number of pages6
JournalMolecular Endocrinology
Volume2
Issue number12
StatePublished - Dec 1 1988
Externally publishedYes

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MCF-7 Cells
Estrogen Receptors
Estradiol
Messenger RNA
Estrogens
Genetic Transcription
Estradiol Receptors
Ribonucleases
Proteins
Down-Regulation
DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Saceda, M., Lippman, M. E., Chambon, P., Lindsey, R. L., Ponglikitmongkol, M., Puente, M., & Martin, M. B. (1988). Regulation of the estrogen receptor in MCF-7 cells by estradiol. Molecular Endocrinology, 2(12), 1157-1162.

Regulation of the estrogen receptor in MCF-7 cells by estradiol. / Saceda, M.; Lippman, Marc E; Chambon, P.; Lindsey, R. L.; Ponglikitmongkol, M.; Puente, M.; Martin, M. B.

In: Molecular Endocrinology, Vol. 2, No. 12, 01.12.1988, p. 1157-1162.

Research output: Contribution to journalArticle

Saceda, M, Lippman, ME, Chambon, P, Lindsey, RL, Ponglikitmongkol, M, Puente, M & Martin, MB 1988, 'Regulation of the estrogen receptor in MCF-7 cells by estradiol', Molecular Endocrinology, vol. 2, no. 12, pp. 1157-1162.
Saceda M, Lippman ME, Chambon P, Lindsey RL, Ponglikitmongkol M, Puente M et al. Regulation of the estrogen receptor in MCF-7 cells by estradiol. Molecular Endocrinology. 1988 Dec 1;2(12):1157-1162.
Saceda, M. ; Lippman, Marc E ; Chambon, P. ; Lindsey, R. L. ; Ponglikitmongkol, M. ; Puente, M. ; Martin, M. B. / Regulation of the estrogen receptor in MCF-7 cells by estradiol. In: Molecular Endocrinology. 1988 ; Vol. 2, No. 12. pp. 1157-1162.
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