Regulation of phospholipase C-γ1 by profilin and tyrosine phosphorylation

Pascal Goldschmidt-Clermont, Jae Won Kim, Laura M. Machesky, Sue Goo Rhee, Thomas D. Pollard

Research output: Contribution to journalArticle

421 Citations (Scopus)

Abstract

Epidermal growth factor and platelet-derived growth factor can stimulate the production of the second messenger inositol trisphosphate in responsive cells, but the biochemical pathway for these signaling events has been uncertain because the reactions have not been reconstituted with purified molecules in vitro. A reconstitution is described that requires not only the growth factor, its receptor with tyrosine kinase activity, and the soluble phospholipase C-γ1, but also the small soluble actin-binding protein profilin. Profilin binds to the substrate phosphatidylinositol 4,5-bisphosphate and inhibits its hydrolysis by unphosphorylated phospholipase C-γ1. Phosphorylation of phospholipase C-γ1 by the epidermal growth factor receptor tyrosine kinase overcomes the inhibitory effect of profilin and results in an effective activation of phospholipase C-γ1.

Original languageEnglish
Pages (from-to)1231-1233
Number of pages3
JournalScience
Volume251
Issue number4998
StatePublished - Mar 8 1991
Externally publishedYes

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Profilins
Type C Phospholipases
Tyrosine
Phosphorylation
Protein-Tyrosine Kinases
Microfilament Proteins
Growth Factor Receptors
Platelet-Derived Growth Factor
Second Messenger Systems
Inositol
Phosphatidylinositols
Epidermal Growth Factor Receptor
Epidermal Growth Factor
Hydrolysis

ASJC Scopus subject areas

  • General

Cite this

Goldschmidt-Clermont, P., Kim, J. W., Machesky, L. M., Rhee, S. G., & Pollard, T. D. (1991). Regulation of phospholipase C-γ1 by profilin and tyrosine phosphorylation. Science, 251(4998), 1231-1233.

Regulation of phospholipase C-γ1 by profilin and tyrosine phosphorylation. / Goldschmidt-Clermont, Pascal; Kim, Jae Won; Machesky, Laura M.; Rhee, Sue Goo; Pollard, Thomas D.

In: Science, Vol. 251, No. 4998, 08.03.1991, p. 1231-1233.

Research output: Contribution to journalArticle

Goldschmidt-Clermont, P, Kim, JW, Machesky, LM, Rhee, SG & Pollard, TD 1991, 'Regulation of phospholipase C-γ1 by profilin and tyrosine phosphorylation', Science, vol. 251, no. 4998, pp. 1231-1233.
Goldschmidt-Clermont P, Kim JW, Machesky LM, Rhee SG, Pollard TD. Regulation of phospholipase C-γ1 by profilin and tyrosine phosphorylation. Science. 1991 Mar 8;251(4998):1231-1233.
Goldschmidt-Clermont, Pascal ; Kim, Jae Won ; Machesky, Laura M. ; Rhee, Sue Goo ; Pollard, Thomas D. / Regulation of phospholipase C-γ1 by profilin and tyrosine phosphorylation. In: Science. 1991 ; Vol. 251, No. 4998. pp. 1231-1233.
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