Regulation of peripheral-type benzodiazepine receptors in cultured astrocytes by monoamine and amino acid neurotransmitters

Yossef Itzhak, Michael D. Norenberg

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Exposure of primary cultured astrocytes for 3 days to 1 μM of either dopamine, serotonin or norepinephrine resulted in upregulation (25-34% increase in Bmax) of the peripheral-type benzodiazepine receptors (PBRs) labeled with [3H]Ro5-4864. A similar treatment with γ-aminobutyric acid [GABA] caused a 2-fold increase in the affinity (Kd) of [3H]Ro5-4864. The monoamines tested and GABA had no effect on the binding parameters of [3H]PK 11195, another selective PBR ligand. The present study indicates that Ro5-4864 binding sites are susceptible to regulation by specific neurotransmitters and provides further evidence for the distinction between Ro5-4864 and PK 11195 binding sites of the PBRs in cultured astrocytes.

Original languageEnglish (US)
Pages (from-to)346-348
Number of pages3
JournalBrain Research
Volume660
Issue number2
DOIs
StatePublished - Oct 17 1994

Keywords

  • Astrocyte
  • culture
  • Dopamine
  • GABA
  • Glutamate
  • Norepinephrine
  • Peripheral benzodiazepine receptor
  • Serotonin

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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