Regulation of perform gene expression in a T cell hybrid with inducible cytolytic activity

José A. García-Sanz, Eckhard R. Podack

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A mouse X rat T cell hybrid (PC60) that does not require interleukin (IL)-2 for proliferation, was used as a model to study regulation of perforin gene expression. Perforin mRNA is barely detectable in non-induced PC60 cells; however, a 30-fold induction is observed after stimulation with IL-1 alone. Peak perforin mRNA levels were reached after 10 h of induction with IL-1, and these levels were maintained for as long as the stimulus was present. IL-2 by itself has no detectable effect. However, in combination with IL-1 it shows the same induction kinetics as IL-1 alone for the first 10 h, subsequently there is synergism (100-fold induction) between IL-1 and IL-2. The induction response was mainly due to increased transcriptional rates of the perforin gene, and require newly synthesized proteins. The half-life of perforin mRNA in this system is about 5 h. In addition, we confirm the existence of two types of mouse perforin mRNA that differ in their 5′ untranslated regions, and show evidence that both mRNA are translated in vivo with similar efficiencies.

Original languageEnglish
Pages (from-to)1877-1883
Number of pages7
JournalEuropean Journal of Immunology
Volume23
Issue number8
StatePublished - Aug 1 1993
Externally publishedYes

Fingerprint

Perforin
Gene Expression Regulation
Interleukin-1
T-Lymphocytes
Messenger RNA
Interleukin-2
5' Untranslated Regions
Half-Life
Genes
Proteins

Keywords

  • Cytotoxic T lymphocytes
  • Gene regulation
  • Perforin

ASJC Scopus subject areas

  • Immunology

Cite this

Regulation of perform gene expression in a T cell hybrid with inducible cytolytic activity. / García-Sanz, José A.; Podack, Eckhard R.

In: European Journal of Immunology, Vol. 23, No. 8, 01.08.1993, p. 1877-1883.

Research output: Contribution to journalArticle

García-Sanz, José A. ; Podack, Eckhard R. / Regulation of perform gene expression in a T cell hybrid with inducible cytolytic activity. In: European Journal of Immunology. 1993 ; Vol. 23, No. 8. pp. 1877-1883.
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