Abstract
Axonal regeneration in the adult CNS is limited by the presence of several inhibitory proteins associated with myelin. Nogo-A, a myelin-associated inhibitor, is responsible for axonal outgrowth inhibition in vivo and in vitro. Here we study the onset and maturation of Nogo-A and Nogo receptor in the entorhino-hippocampal formation of developing and adult mice. We also provide evidence that Nogo-A does not inhibit embryonic hippocampal neurons, in contrast to other cell types such as cerebellar granule cells. Our results also show that Nogo and Nogo receptor mRNA are expressed in the adult by both principal and local-circuit hippocampal neurons, and that after lesion, Nogo-A is also transiently expressed by a subset of reactive astrocytes. Furthermore, we analyzed their regulation after kainic acid (KA) treatment and in response to the transection of the entorhino-hippocampal connection. We found that Nogo-A and Nogo receptor are differentially regulated after kainic acid or perforant pathway lesions. Lastly, we show that the regenerative potential of lesioned entorhino-hippocampal organotypic slice co-cultures is increased after blockage of Nogo-A with two IN-1 blocking antibodies. In conclusion, our results show that Nogo and its receptor might play key roles during development of hippocampal connections and that they are implicated in neuronal plasticity in the adult.
| Original language | English |
|---|---|
| Pages (from-to) | 34-49 |
| Number of pages | 16 |
| Journal | Molecular and Cellular Neuroscience |
| Volume | 26 |
| Issue number | 1 |
| DOIs | |
| State | Published - May 1 2004 |
Fingerprint
ASJC Scopus subject areas
- Molecular Biology
- Cellular and Molecular Neuroscience
- Developmental Neuroscience
Cite this
Regulation of Nogo and Nogo receptor during the development of the entorhino-hippocampal pathway and after adult hippocampal lesions. / Mingorance, Ana; Fontana, Xavier; Solé, Marta; Burgaya, Ferran; Ureña, Jesús M.; Teng, Felicia Y H; Tang, Bor Luen; Hunt, David; Anderson, Patrick N.; Bethea, John R.; Schwab, Martin E.; Soriano, Eduardo; Del Río, José A.
In: Molecular and Cellular Neuroscience, Vol. 26, No. 1, 01.05.2004, p. 34-49.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Regulation of Nogo and Nogo receptor during the development of the entorhino-hippocampal pathway and after adult hippocampal lesions
AU - Mingorance, Ana
AU - Fontana, Xavier
AU - Solé, Marta
AU - Burgaya, Ferran
AU - Ureña, Jesús M.
AU - Teng, Felicia Y H
AU - Tang, Bor Luen
AU - Hunt, David
AU - Anderson, Patrick N.
AU - Bethea, John R.
AU - Schwab, Martin E.
AU - Soriano, Eduardo
AU - Del Río, José A.
PY - 2004/5/1
Y1 - 2004/5/1
N2 - Axonal regeneration in the adult CNS is limited by the presence of several inhibitory proteins associated with myelin. Nogo-A, a myelin-associated inhibitor, is responsible for axonal outgrowth inhibition in vivo and in vitro. Here we study the onset and maturation of Nogo-A and Nogo receptor in the entorhino-hippocampal formation of developing and adult mice. We also provide evidence that Nogo-A does not inhibit embryonic hippocampal neurons, in contrast to other cell types such as cerebellar granule cells. Our results also show that Nogo and Nogo receptor mRNA are expressed in the adult by both principal and local-circuit hippocampal neurons, and that after lesion, Nogo-A is also transiently expressed by a subset of reactive astrocytes. Furthermore, we analyzed their regulation after kainic acid (KA) treatment and in response to the transection of the entorhino-hippocampal connection. We found that Nogo-A and Nogo receptor are differentially regulated after kainic acid or perforant pathway lesions. Lastly, we show that the regenerative potential of lesioned entorhino-hippocampal organotypic slice co-cultures is increased after blockage of Nogo-A with two IN-1 blocking antibodies. In conclusion, our results show that Nogo and its receptor might play key roles during development of hippocampal connections and that they are implicated in neuronal plasticity in the adult.
AB - Axonal regeneration in the adult CNS is limited by the presence of several inhibitory proteins associated with myelin. Nogo-A, a myelin-associated inhibitor, is responsible for axonal outgrowth inhibition in vivo and in vitro. Here we study the onset and maturation of Nogo-A and Nogo receptor in the entorhino-hippocampal formation of developing and adult mice. We also provide evidence that Nogo-A does not inhibit embryonic hippocampal neurons, in contrast to other cell types such as cerebellar granule cells. Our results also show that Nogo and Nogo receptor mRNA are expressed in the adult by both principal and local-circuit hippocampal neurons, and that after lesion, Nogo-A is also transiently expressed by a subset of reactive astrocytes. Furthermore, we analyzed their regulation after kainic acid (KA) treatment and in response to the transection of the entorhino-hippocampal connection. We found that Nogo-A and Nogo receptor are differentially regulated after kainic acid or perforant pathway lesions. Lastly, we show that the regenerative potential of lesioned entorhino-hippocampal organotypic slice co-cultures is increased after blockage of Nogo-A with two IN-1 blocking antibodies. In conclusion, our results show that Nogo and its receptor might play key roles during development of hippocampal connections and that they are implicated in neuronal plasticity in the adult.
UR - http://www.scopus.com/inward/record.url?scp=2342593377&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2342593377&partnerID=8YFLogxK
U2 - 10.1016/j.mcn.2004.01.001
DO - 10.1016/j.mcn.2004.01.001
M3 - Article
C2 - 15121177
AN - SCOPUS:2342593377
VL - 26
SP - 34
EP - 49
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
SN - 1044-7431
IS - 1
ER -