Regulation of Nogo and Nogo receptor during the development of the entorhino-hippocampal pathway and after adult hippocampal lesions

Ana Mingorance, Xavier Fontana, Marta Solé, Ferran Burgaya, Jesús M. Ureña, Felicia Y H Teng, Bor Luen Tang, David Hunt, Patrick N. Anderson, John R. Bethea, Martin E. Schwab, Eduardo Soriano, José A. Del Río

Research output: Contribution to journalArticle

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Abstract

Axonal regeneration in the adult CNS is limited by the presence of several inhibitory proteins associated with myelin. Nogo-A, a myelin-associated inhibitor, is responsible for axonal outgrowth inhibition in vivo and in vitro. Here we study the onset and maturation of Nogo-A and Nogo receptor in the entorhino-hippocampal formation of developing and adult mice. We also provide evidence that Nogo-A does not inhibit embryonic hippocampal neurons, in contrast to other cell types such as cerebellar granule cells. Our results also show that Nogo and Nogo receptor mRNA are expressed in the adult by both principal and local-circuit hippocampal neurons, and that after lesion, Nogo-A is also transiently expressed by a subset of reactive astrocytes. Furthermore, we analyzed their regulation after kainic acid (KA) treatment and in response to the transection of the entorhino-hippocampal connection. We found that Nogo-A and Nogo receptor are differentially regulated after kainic acid or perforant pathway lesions. Lastly, we show that the regenerative potential of lesioned entorhino-hippocampal organotypic slice co-cultures is increased after blockage of Nogo-A with two IN-1 blocking antibodies. In conclusion, our results show that Nogo and its receptor might play key roles during development of hippocampal connections and that they are implicated in neuronal plasticity in the adult.

Original languageEnglish
Pages (from-to)34-49
Number of pages16
JournalMolecular and Cellular Neuroscience
Volume26
Issue number1
DOIs
StatePublished - May 1 2004

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Kainic Acid
Myelin Sheath
Perforant Pathway
Neurons
Neuronal Plasticity
Blocking Antibodies
Coculture Techniques
Astrocytes
Regeneration
Hippocampus
Messenger RNA
Nogo Receptors
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Regulation of Nogo and Nogo receptor during the development of the entorhino-hippocampal pathway and after adult hippocampal lesions. / Mingorance, Ana; Fontana, Xavier; Solé, Marta; Burgaya, Ferran; Ureña, Jesús M.; Teng, Felicia Y H; Tang, Bor Luen; Hunt, David; Anderson, Patrick N.; Bethea, John R.; Schwab, Martin E.; Soriano, Eduardo; Del Río, José A.

In: Molecular and Cellular Neuroscience, Vol. 26, No. 1, 01.05.2004, p. 34-49.

Research output: Contribution to journalArticle

Mingorance, A, Fontana, X, Solé, M, Burgaya, F, Ureña, JM, Teng, FYH, Tang, BL, Hunt, D, Anderson, PN, Bethea, JR, Schwab, ME, Soriano, E & Del Río, JA 2004, 'Regulation of Nogo and Nogo receptor during the development of the entorhino-hippocampal pathway and after adult hippocampal lesions', Molecular and Cellular Neuroscience, vol. 26, no. 1, pp. 34-49. https://doi.org/10.1016/j.mcn.2004.01.001
Mingorance, Ana ; Fontana, Xavier ; Solé, Marta ; Burgaya, Ferran ; Ureña, Jesús M. ; Teng, Felicia Y H ; Tang, Bor Luen ; Hunt, David ; Anderson, Patrick N. ; Bethea, John R. ; Schwab, Martin E. ; Soriano, Eduardo ; Del Río, José A. / Regulation of Nogo and Nogo receptor during the development of the entorhino-hippocampal pathway and after adult hippocampal lesions. In: Molecular and Cellular Neuroscience. 2004 ; Vol. 26, No. 1. pp. 34-49.
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