@article{0b1759c5da1f467d846ad49c35e17338,
title = "Regulation of Nogo and Nogo receptor during the development of the entorhino-hippocampal pathway and after adult hippocampal lesions",
abstract = "Axonal regeneration in the adult CNS is limited by the presence of several inhibitory proteins associated with myelin. Nogo-A, a myelin-associated inhibitor, is responsible for axonal outgrowth inhibition in vivo and in vitro. Here we study the onset and maturation of Nogo-A and Nogo receptor in the entorhino-hippocampal formation of developing and adult mice. We also provide evidence that Nogo-A does not inhibit embryonic hippocampal neurons, in contrast to other cell types such as cerebellar granule cells. Our results also show that Nogo and Nogo receptor mRNA are expressed in the adult by both principal and local-circuit hippocampal neurons, and that after lesion, Nogo-A is also transiently expressed by a subset of reactive astrocytes. Furthermore, we analyzed their regulation after kainic acid (KA) treatment and in response to the transection of the entorhino-hippocampal connection. We found that Nogo-A and Nogo receptor are differentially regulated after kainic acid or perforant pathway lesions. Lastly, we show that the regenerative potential of lesioned entorhino-hippocampal organotypic slice co-cultures is increased after blockage of Nogo-A with two IN-1 blocking antibodies. In conclusion, our results show that Nogo and its receptor might play key roles during development of hippocampal connections and that they are implicated in neuronal plasticity in the adult.",
author = "Ana Mingorance and Xavier Fontana and Marta Sol{\'e} and Ferran Burgaya and Ure{\~n}a, {Jes{\'u}s M.} and Teng, {Felicia Y.H.} and Tang, {Bor Luen} and David Hunt and Anderson, {Patrick N.} and Bethea, {John R.} and Schwab, {Martin E.} and Eduardo Soriano and {Del R{\'i}o}, {Jos{\'e} A.}",
note = "Funding Information: We thank Prof. Y. Tsujimoto (Department of Medical Genetics, Osaka University Graduate School of Medicine, Osaka, Japan) for providing the HA-tagged Nogo-A expression vector, Dr. Z. He (Harvard Medical School, Longwood Avenue, MA, USA) for providing the AP-Nogo-66 construction, and Dr. M. Yutsudo (Research Institute for Microbial Diseases, Osaka University, Osaka, Japan) for providing the Nogo-B cDNA. The authors also thank Tatsumi Hirata (National Institute of Genetics, Graduate University for Advanced Studies, Mishima, Japan) for the gift of the NG1 antibody and Nathalia Vitureira and Anna La Torre for their technical advice on the non-radioactive in situ hybridization and preparation of the mRNA probes. The authors are also grateful to Susana Maqueda for her technical assistance and Robin Rycroft for linguistic advice. This study was supported by grants from the Spanish Ministry of Science and Technology (MCYT) (EET2003-05149 and BFI2003-03594), FISS (FIS01-0895) and The Caixa Foundation (2004–2007) to JADR, and MCYT (SAF2001-3290) to E.S. Ana Mingorance is a fellow of the Universitat de Barcelona and Xavier Fontana is supplied by fellows from the Fundaci{\'o} Agust{\'ı} Pedro i Pons (2001–2002) and the Spanish Ministry of Education and Culture (2003).",
year = "2004",
month = may,
doi = "10.1016/j.mcn.2004.01.001",
language = "English (US)",
volume = "26",
pages = "34--49",
journal = "Molecular and Cellular Neuroscience",
issn = "1044-7431",
publisher = "Academic Press Inc.",
number = "1",
}