Regulation of MEF2 transcriptional activity by calcineurin/mAKAP complexes

Jinliang Li, Maximilian A.X. Vargas, Michael S. Kapiloff, Kimberly L. Dodge-Kafka

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The calcium/calmodulin-dependent protein phosphatase calcineurin is required for the induction of transcriptional events that initiate and promote myogenic differentiation. An important effector for calcineurin in striated muscle is the transcription factor myocyte enhancer factor 2 (MEF2). The targeting of the enzyme and substrate to specific intracellular compartments by scaffold proteins often confers specificity in phosphatase activity. We now show that the scaffolding protein mAKAP organizes a calcineurin/MEF2 signaling complex in myocytes, regulating gene transcription. A calcineurin/mAKAP/MEF2 complex can be isolated from C2C12 cells and cardiac myocytes, and the calcineurin/MEF2 association is dependent on mAKAP expression. We have identified a peptide comprising the calcineurin binding domain in mAKAP that can disrupt the binding of the phosphatase to the scaffold in vivo. Dominant interference of calcineurin/mAKAP binding blunts the increase in MEF2 transcriptional activity seen during myoblast differentiation, as well as the expression of endogenous MEF2-target genes. Furthermore, disruption of calcineurin binding to mAKAP in cardiac myocytes inhibits adrenergic-induced cellular hypertrophy. Together these data illustrate the importance of calcineurin anchoring by the mAKAP scaffold for MEF2 regulation.

Original languageEnglish (US)
Pages (from-to)447-454
Number of pages8
JournalExperimental Cell Research
Volume319
Issue number4
DOIs
StatePublished - Feb 15 2013

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Keywords

  • AKAP
  • Calcineurin
  • MEF2
  • Protein phosphatase
  • Scaffold

ASJC Scopus subject areas

  • Cell Biology

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