Regulation of HTLV-II gene expression by rex involves positive and negative cis-acting elements in the 5′ long terminal repeat

Alexander C. Black, Irvin Sy Chen, Salvatore Arrigo, Cristina T. Ruland, Thomas Allogiamento, Eva Chin, Joseph D Rosenblatt

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Regulation of human T-cell leukemia virus type II (HTLV-II) gene expression by the trans-acting viral protein, Rex, is mediated through specific cis-acting sequences in the HTLV-II long terminal repeat (LTR). Augmentation of 5′ LTR-linked gene expression by Rex requires two distinct cis-acting elements: one termed the "Rex-responsive element" (RxRE), which allows Rex to overcome the inhibitory effect of a second, termed the "cis-acting repressive sequences" (CRS). The HTLV-II RxRE is located between nt +91 and +317 relative to the cap site in the R/U5 region of the 5′ LTR, and the HTLV-II CRS is contained within the RxRE from nt +208 to +317, which is downstream of the splice donor site, in the R/U5 region of the 5′ LTR. Deletion of the CRS results in significantly increased cytoplasmic levels of LTR-linked mRNA independent of the presence of Rex. Our results show that Rex acts post-transcriptionally and induces a shift from nucleus to cytoplasm of gag/pol mRNA, the only HTLV-II mRNA that contains both the RxRE and the CRS in the 5′ LTR-derived leader sequence.

Original languageEnglish
Pages (from-to)433-444
Number of pages12
JournalVirology
Volume181
Issue number2
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

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Human T-lymphotropic virus 2
Terminal Repeat Sequences
Gene Expression
Messenger RNA
RNA Splice Sites
Viral Proteins
Cytoplasm

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Regulation of HTLV-II gene expression by rex involves positive and negative cis-acting elements in the 5′ long terminal repeat. / Black, Alexander C.; Chen, Irvin Sy; Arrigo, Salvatore; Ruland, Cristina T.; Allogiamento, Thomas; Chin, Eva; Rosenblatt, Joseph D.

In: Virology, Vol. 181, No. 2, 01.01.1991, p. 433-444.

Research output: Contribution to journalArticle

Black, Alexander C. ; Chen, Irvin Sy ; Arrigo, Salvatore ; Ruland, Cristina T. ; Allogiamento, Thomas ; Chin, Eva ; Rosenblatt, Joseph D. / Regulation of HTLV-II gene expression by rex involves positive and negative cis-acting elements in the 5′ long terminal repeat. In: Virology. 1991 ; Vol. 181, No. 2. pp. 433-444.
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