Regulation of glycolytic enzyme RNA transcriptional rates by oxygen availability in skeletal muscle cells

Keith A Webster

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Cytoplasmic β-actin and five glycolytic enzyme cDNAs were isolated from a rat skeletal muscle cDNA library and together with a genomic clone of rat cytochrome c were used as probes to quantitate the respective RNA transcription rates in isolated nuclei run off transcription assays from stationary cells cultured under normal or 2% oxygen. The transcription rates of lactate dehydrogenase, pyruvate kinase, triosephosphate isomerase and aldolase increased by 2-5 fold during the 72 hr exposure to 2% oxygen. There was a small increase in actin RNA transcription while both cytochrome c and glyceraldehyde-3-phosphate dehydrogenase RNA transcription rates decreased. Since previous studies demonstrated an increase in steady state glyceraldehyde-3-phosphate dehydrogenase RNA during low Oz exposure it is concluded that the level of this RNA is regulated post transcriptionally whereas the other four glycolytic enzyme RNAs are regulated at least partially at the level of transcription by oxygen availability. The relative transcriptional rates of the RNAs in this study are related to their cellular RNA and protein concentrations.

Original languageEnglish
Pages (from-to)19-28
Number of pages10
JournalMolecular and Cellular Biochemistry
Volume77
Issue number1
DOIs
StatePublished - Mar 1 1987
Externally publishedYes

Fingerprint

Muscle Cells
Muscle
Skeletal Muscle
Transcription
Cells
Availability
RNA
Oxygen
Enzymes
Glyceraldehyde-3-Phosphate Dehydrogenases
Cytochromes c
Rats
Actins
Triose-Phosphate Isomerase
Fructose-Bisphosphate Aldolase
Pyruvate Kinase
Gene Library
L-Lactate Dehydrogenase
Cultured Cells
Assays

Keywords

  • glycolysis
  • myoblast
  • oxygen regulation
  • transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Clinical Biochemistry
  • Cell Biology

Cite this

Regulation of glycolytic enzyme RNA transcriptional rates by oxygen availability in skeletal muscle cells. / Webster, Keith A.

In: Molecular and Cellular Biochemistry, Vol. 77, No. 1, 01.03.1987, p. 19-28.

Research output: Contribution to journalArticle

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