Regulation of expression of human granulocyte/macrophage colony-stimulating factor

J. Y. Chan, D. J. Slamon, Stephen D Nimer, D. W. Golde, J. C. Gasson

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Colony-stimulating factors (CSFs) are glycoproteins that stimulate the growth of hematopoietic progenitors and enhance the functional activity of mature effector cells. Human granulocyte/macrophage colony-stimulating factor (GM-CSF) is a 22-kDa glycoprotein that stimulates the growth of myeloid and erythroid progenitors in vitro and increases the responsiveness of neutrophils, monocytes, and eosinophils to physiologic stimuli. Elucidation of the cell and tissue sources of CSFs, as well as study of their regulation of expression, is required to understand their role in physiologic and pathophysiologic states. An extensive survey of normal and neoplastic human tissues did not reveal constitutive production of detectable levels of GM-CSF mRNA in any of the 64 samples studied. Antigen- or lectin-activated T lymphocytes have been shown to produce GM-CSF; therefore, to elucidate the genetic sequences required, we constructed recombinant plasmids containing 5' flanking DNA of the GM-CSF gene linked to the marker chloramphenicol acetyltransferase gene. The recombinant constructs were transfected into a human T-cell leukemia virus type I (HTLV)-infected T-lymphoblast cell line that can be stimulated to produce high levels of GM-CSF. We show here that the 5' flanking sequences of the GM-CSF gene can direct increased expression of the chloramphenicol acetyltransferase gene in activated T-lymphoblast cells.

Original languageEnglish
Pages (from-to)8669-8673
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number22
DOIs
StatePublished - Dec 1 1986
Externally publishedYes

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Granulocyte-Macrophage Colony-Stimulating Factor
Colony-Stimulating Factors
Chloramphenicol O-Acetyltransferase
Genes
Glycoproteins
Human T-lymphotropic virus 1
5' Flanking Region
Growth
Eosinophils
Lectins
Monocytes
Neutrophils
Plasmids
T-Lymphocytes
Antigens
Cell Line
Messenger RNA
DNA

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Regulation of expression of human granulocyte/macrophage colony-stimulating factor. / Chan, J. Y.; Slamon, D. J.; Nimer, Stephen D; Golde, D. W.; Gasson, J. C.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 22, 01.12.1986, p. 8669-8673.

Research output: Contribution to journalArticle

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