Regulation of endothelial cell adhesion by profilin

Nicanor I. Moldovan, Emily E. Milliken, Kaikobad Irani, Jie Chen, Richard H. Sohn, Toren Finkel, Pascal Goldschmidt-Clermont

Research output: Contribution to journalArticle

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Abstract

Background: Although profilin is believed to be an essential regulator of the actin cytoskeleton in most cells, its precise role in mammalian cells remains unknown. We have used replication-incompetent adenovirus carrying the human profilin I cDNA as a means rapidly to increase the concentration of profilin in human aortic endothelial cells 12-31 -fold above baseline - levels never before achieved in mammalian cells. Results: The concentration of filamentous actin was not detectably affected by profilin overexpression. Actin stress fibers were, however, absent from areas of high profilin content in overexpressing cells, and the bulk of filaments was located at the periphery of the cells. We observed a gradient in the distribution of overexpressed profilin in migrating endothelial cells, with most profilin molecules concentrated near the advancing edge where focal contacts are being formed and focal adhesion proteins are located. Profilin overexpression resulted in increased recruitment of fibronectin receptors to the plasma membrane. Adhesion of endothelial cells to fibronectin was markedly and selectively increased by profilin overexpression. Conclusions: We conclude that an important role for profilin in mammalian cells may be its contribution to the formation of focal contacts, particularly those involving the fibronectin receptor.

Original languageEnglish
Pages (from-to)24-30
Number of pages7
JournalCurrent Biology
Volume7
Issue number1
StatePublished - Jan 1 1997
Externally publishedYes

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Profilins
Cell adhesion
Endothelial cells
cell adhesion
Cell Adhesion
endothelial cells
Endothelial Cells
Focal Adhesions
fibronectins
Actins
Cells
cells
actin
adhesion
Fibronectin Receptors
Adhesion
profilins
Integrin alpha5beta1
Human Adenoviruses
Stress Fibers

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Moldovan, N. I., Milliken, E. E., Irani, K., Chen, J., Sohn, R. H., Finkel, T., & Goldschmidt-Clermont, P. (1997). Regulation of endothelial cell adhesion by profilin. Current Biology, 7(1), 24-30.

Regulation of endothelial cell adhesion by profilin. / Moldovan, Nicanor I.; Milliken, Emily E.; Irani, Kaikobad; Chen, Jie; Sohn, Richard H.; Finkel, Toren; Goldschmidt-Clermont, Pascal.

In: Current Biology, Vol. 7, No. 1, 01.01.1997, p. 24-30.

Research output: Contribution to journalArticle

Moldovan, NI, Milliken, EE, Irani, K, Chen, J, Sohn, RH, Finkel, T & Goldschmidt-Clermont, P 1997, 'Regulation of endothelial cell adhesion by profilin', Current Biology, vol. 7, no. 1, pp. 24-30.
Moldovan NI, Milliken EE, Irani K, Chen J, Sohn RH, Finkel T et al. Regulation of endothelial cell adhesion by profilin. Current Biology. 1997 Jan 1;7(1):24-30.
Moldovan, Nicanor I. ; Milliken, Emily E. ; Irani, Kaikobad ; Chen, Jie ; Sohn, Richard H. ; Finkel, Toren ; Goldschmidt-Clermont, Pascal. / Regulation of endothelial cell adhesion by profilin. In: Current Biology. 1997 ; Vol. 7, No. 1. pp. 24-30.
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