Regulation of dendrite growth and maintenance by exocytosis

Yun Peng, Jiae Lee, Kimberly Rowland, Yuhui Wen, Hope Hua, Nicole Carlson, Shweta Lavania, Jay Z. Parrish, Michael D. Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Dendrites lengthen by several orders of magnitude during neuronal development, but how membrane is allocated in dendrites to facilitate this growth remains unclear. Here, we report that Ras opposite (Rop), the Drosophila ortholog of the key exocytosis regulator Munc18-1 (also known as STXBP1), is an essential factor mediating dendrite growth. Neurons with depleted Rop function exhibit reduced terminal dendrite outgrowth followed by primary dendrite degeneration, suggestive of differential requirements for exocytosis in the growth and maintenance of different dendritic compartments. Rop promotes dendrite growth together with the exocyst, an octameric protein complex involved in tethering vesicles to the plasma membrane, with Rop-exocyst complexes and exocytosis predominating in primary dendrites over terminal dendrites. By contrast, membrane-associated proteins readily diffuse from primary dendrites into terminals, but not in the reverse direction, suggesting that diffusion, rather than targeted exocytosis, supplies membranous material for terminal dendritic growth, revealing key differences in the distribution of materials to these expanding dendritic compartments.

Original languageEnglish (US)
Pages (from-to)4279-4292
Number of pages14
JournalJournal of Cell Science
Volume128
Issue number23
DOIs
StatePublished - 2015
Externally publishedYes

Fingerprint

Exocytosis
Dendrites
Maintenance
Growth
Drosophila
Membrane Proteins
Cell Membrane
Neurons
Membranes

Keywords

  • Dendrite
  • Drosophila
  • Exocyst
  • Munc18-1
  • Neurodegeneration
  • STXBP1

ASJC Scopus subject areas

  • Cell Biology

Cite this

Peng, Y., Lee, J., Rowland, K., Wen, Y., Hua, H., Carlson, N., ... Kim, M. D. (2015). Regulation of dendrite growth and maintenance by exocytosis. Journal of Cell Science, 128(23), 4279-4292. https://doi.org/10.1242/jcs.174771

Regulation of dendrite growth and maintenance by exocytosis. / Peng, Yun; Lee, Jiae; Rowland, Kimberly; Wen, Yuhui; Hua, Hope; Carlson, Nicole; Lavania, Shweta; Parrish, Jay Z.; Kim, Michael D.

In: Journal of Cell Science, Vol. 128, No. 23, 2015, p. 4279-4292.

Research output: Contribution to journalArticle

Peng, Y, Lee, J, Rowland, K, Wen, Y, Hua, H, Carlson, N, Lavania, S, Parrish, JZ & Kim, MD 2015, 'Regulation of dendrite growth and maintenance by exocytosis', Journal of Cell Science, vol. 128, no. 23, pp. 4279-4292. https://doi.org/10.1242/jcs.174771
Peng Y, Lee J, Rowland K, Wen Y, Hua H, Carlson N et al. Regulation of dendrite growth and maintenance by exocytosis. Journal of Cell Science. 2015;128(23):4279-4292. https://doi.org/10.1242/jcs.174771
Peng, Yun ; Lee, Jiae ; Rowland, Kimberly ; Wen, Yuhui ; Hua, Hope ; Carlson, Nicole ; Lavania, Shweta ; Parrish, Jay Z. ; Kim, Michael D. / Regulation of dendrite growth and maintenance by exocytosis. In: Journal of Cell Science. 2015 ; Vol. 128, No. 23. pp. 4279-4292.
@article{8f12418fc31645adb537f8a053acface,
title = "Regulation of dendrite growth and maintenance by exocytosis",
abstract = "Dendrites lengthen by several orders of magnitude during neuronal development, but how membrane is allocated in dendrites to facilitate this growth remains unclear. Here, we report that Ras opposite (Rop), the Drosophila ortholog of the key exocytosis regulator Munc18-1 (also known as STXBP1), is an essential factor mediating dendrite growth. Neurons with depleted Rop function exhibit reduced terminal dendrite outgrowth followed by primary dendrite degeneration, suggestive of differential requirements for exocytosis in the growth and maintenance of different dendritic compartments. Rop promotes dendrite growth together with the exocyst, an octameric protein complex involved in tethering vesicles to the plasma membrane, with Rop-exocyst complexes and exocytosis predominating in primary dendrites over terminal dendrites. By contrast, membrane-associated proteins readily diffuse from primary dendrites into terminals, but not in the reverse direction, suggesting that diffusion, rather than targeted exocytosis, supplies membranous material for terminal dendritic growth, revealing key differences in the distribution of materials to these expanding dendritic compartments.",
keywords = "Dendrite, Drosophila, Exocyst, Munc18-1, Neurodegeneration, STXBP1",
author = "Yun Peng and Jiae Lee and Kimberly Rowland and Yuhui Wen and Hope Hua and Nicole Carlson and Shweta Lavania and Parrish, {Jay Z.} and Kim, {Michael D.}",
year = "2015",
doi = "10.1242/jcs.174771",
language = "English (US)",
volume = "128",
pages = "4279--4292",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "23",

}

TY - JOUR

T1 - Regulation of dendrite growth and maintenance by exocytosis

AU - Peng, Yun

AU - Lee, Jiae

AU - Rowland, Kimberly

AU - Wen, Yuhui

AU - Hua, Hope

AU - Carlson, Nicole

AU - Lavania, Shweta

AU - Parrish, Jay Z.

AU - Kim, Michael D.

PY - 2015

Y1 - 2015

N2 - Dendrites lengthen by several orders of magnitude during neuronal development, but how membrane is allocated in dendrites to facilitate this growth remains unclear. Here, we report that Ras opposite (Rop), the Drosophila ortholog of the key exocytosis regulator Munc18-1 (also known as STXBP1), is an essential factor mediating dendrite growth. Neurons with depleted Rop function exhibit reduced terminal dendrite outgrowth followed by primary dendrite degeneration, suggestive of differential requirements for exocytosis in the growth and maintenance of different dendritic compartments. Rop promotes dendrite growth together with the exocyst, an octameric protein complex involved in tethering vesicles to the plasma membrane, with Rop-exocyst complexes and exocytosis predominating in primary dendrites over terminal dendrites. By contrast, membrane-associated proteins readily diffuse from primary dendrites into terminals, but not in the reverse direction, suggesting that diffusion, rather than targeted exocytosis, supplies membranous material for terminal dendritic growth, revealing key differences in the distribution of materials to these expanding dendritic compartments.

AB - Dendrites lengthen by several orders of magnitude during neuronal development, but how membrane is allocated in dendrites to facilitate this growth remains unclear. Here, we report that Ras opposite (Rop), the Drosophila ortholog of the key exocytosis regulator Munc18-1 (also known as STXBP1), is an essential factor mediating dendrite growth. Neurons with depleted Rop function exhibit reduced terminal dendrite outgrowth followed by primary dendrite degeneration, suggestive of differential requirements for exocytosis in the growth and maintenance of different dendritic compartments. Rop promotes dendrite growth together with the exocyst, an octameric protein complex involved in tethering vesicles to the plasma membrane, with Rop-exocyst complexes and exocytosis predominating in primary dendrites over terminal dendrites. By contrast, membrane-associated proteins readily diffuse from primary dendrites into terminals, but not in the reverse direction, suggesting that diffusion, rather than targeted exocytosis, supplies membranous material for terminal dendritic growth, revealing key differences in the distribution of materials to these expanding dendritic compartments.

KW - Dendrite

KW - Drosophila

KW - Exocyst

KW - Munc18-1

KW - Neurodegeneration

KW - STXBP1

UR - http://www.scopus.com/inward/record.url?scp=84949818003&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84949818003&partnerID=8YFLogxK

U2 - 10.1242/jcs.174771

DO - 10.1242/jcs.174771

M3 - Article

AN - SCOPUS:84949818003

VL - 128

SP - 4279

EP - 4292

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 23

ER -