Regulation of cortical cyclooxygenase-2 expression in rats with subtotal renal ablation

Wei Xin Kong, Yong Gu, Ji Ma, Yi Qin Zuo, Hai Chun Yang, Shan Yan Lin

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1 Scopus citations

Abstract

Purpose: To investigate the regulation of cortical cyclooxygenase-2 expression in rats with subtotal renal ablation. Methods: Rats were ran domly assigned to six groups with 6 animals in each group:sham-operated (Sham), subtotal renal ablation(SNX), SNX treated with rofecoxib (10 mg/kg · d-1), SNX treated with indomethacin (2 mg/kg · d -1), SNX treated with losartan (100 mg/kg · d-1), SNX treated with rofecoxib and losartan (R + L). The urinary protein and thromboxane B2 as well as cortical angiotensin II were measured after six weeks. The expression of cortical COX2 and COX1 were detected by way of immunohistochemistry, RT-PCR and Western blotting. Results: Systolic BP was significantly increased in group SNX 6 weeks after subtotal renal ablation. The increases in SBP were significantly blunted in group Los and group R + L (P < 0.05), while SBP in group Rof and group Ind rats were not significantly different from that in SNX. The rats in group SNX manifested progressively increasing proteinuria (101.7 ± 28.4)mg/d and COX2 expression and TXB2 excretion were significantly increased while COX1 expression remained undisturbed in comparison with those in Sham group. The level of angiotensin II in cortex was increased in group SNX. Rofecoxib significantly down-regulated COX2 expression accompanied with TXB2 excretion decreased while COX1 expression remained undistubed. Indomethacin significantly decreased both of COX2 and COX1. Conclusions: COX2 expression and TXB2 excretion were significantly increased in rats with subtotal renal ablation while COX1 expression remained undistubed. Rofecoxib down-regulated COX2 expression and partly inhibited the activation of renal renin-angiotensin system.

Original languageEnglish (US)
Pages (from-to)13-17
Number of pages5
JournalFudan University Journal of Medical Sciences
Volume32
Issue number1
StatePublished - Jan 2005
Externally publishedYes

Keywords

  • Cyclooxygenase-2
  • Macula densa
  • Subtotal renal ablation

ASJC Scopus subject areas

  • Medicine(all)

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