Regulation of Ca2+-activated tension in limulus striated muscle

W. Glenn Kerrick, L. L. Bolles

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Skinned tail and leg muscle fibers of the limulus were used to study the mechanism of Ca2+ regulation of contraction. Although a Ca2+-sensitive 31,000 dalton protein phosphorylation could be observed in the presence of [γ-32P] ATP no such phosphorylation occurred in the presence of [γ-32P] ITP. Ca2+-activated tension occurred equally as well in ATP and ITP. For this reason we eliminated the possibility that a Ca2+-sensitive myosin light chain kinase/phosphatase system is the mechanism responsible for the Ca2+-activated tension. Other agents known to affect a myosin light chain kinase/phosphatase system showed negative results (ATPγS, trifluoperazine, catalytic subunit of the cyclic adenosine 3′,5′-monophosphate dependent protein kinase and calmodulin). Troponin I reversibly inhibits Ca2+-activated tension. These results are consistent with thin filament regulation being responsible for Ca2+-activated tension in skinned fibers.

Original languageEnglish
Pages (from-to)121-124
Number of pages4
JournalPflügers Archiv European Journal of Physiology
Volume392
Issue number2
DOIs
StatePublished - Dec 1 1981
Externally publishedYes

Fingerprint

Myosin-Light-Chain Phosphatase
Inosine Triphosphate
Myosin-Light-Chain Kinase
Horseshoe Crabs
Phosphorylation
Striated Muscle
Phosphoric Monoester Hydrolases
Muscle
Adenosine Triphosphate
Trifluoperazine
Calcium-Calmodulin-Dependent Protein Kinases
Troponin I
Fibers
Adenosine
Tail
Catalytic Domain
Leg
Muscles
Proteins

Keywords

  • Ca
  • ITP
  • Limulus
  • Myosin light chain
  • Phosphorylation
  • Skinned fibers

ASJC Scopus subject areas

  • Physiology

Cite this

Regulation of Ca2+-activated tension in limulus striated muscle. / Kerrick, W. Glenn; Bolles, L. L.

In: Pflügers Archiv European Journal of Physiology, Vol. 392, No. 2, 01.12.1981, p. 121-124.

Research output: Contribution to journalArticle

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