Regulation of caspases and XIAP in the brain after asphyxial cardiac arrest in rats

Laurence M. Katz, George Lotocki, Yuanfan Wang, Susan Kraydieh, W. Dalton Dietrich, Robert W. Keane

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

The aim of this study was to determine whether hypoxic-ischemia from asphyxial cardiac arrest activates brain caspases-1 and -3, and the anti-apoptotic protein, XIAP. Asphyxial cardiac arrest in rats was used to induce hypoxic-ischemia. A pan-caspase inhibitor (zVAD) was given in the treatment group. At 72 h after reperfusion, caspase-3 and XIAP expression were present in multiple vulnerable brain regions, whereas caspase-1 was predominantly found in the CAI hippocampus. zVAD significantly reduced expression of caspases and XIAP and the number of ischemic neurons in the CAI hippocampus while neurological deficit scores were improved. We conclude that hypoxic-ischemia increases caspases-1 and-3, and XIAP expression. Treatment with zVAD significantly decreases caspase and XIAP expression in these brain regions and improves neurological outcome.

Original languageEnglish (US)
Pages (from-to)3751-3754
Number of pages4
JournalNeuroreport
Volume12
Issue number17
DOIs
StatePublished - Dec 4 2001

Keywords

  • Cardiac arrest
  • Caspase
  • Hypoxia
  • Ischemia

ASJC Scopus subject areas

  • Neuroscience(all)

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