Regulation of caspases and XIAP in the brain after asphyxial cardiac arrest in rats

Laurence M. Katz, George Lotocki, Yuanfan Wang, Susan Kraydieh, W. Dalton Dietrich, Robert Keane

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The aim of this study was to determine whether hypoxic-ischemia from asphyxial cardiac arrest activates brain caspases-1 and -3, and the anti-apoptotic protein, XIAP. Asphyxial cardiac arrest in rats was used to induce hypoxic-ischemia. A pan-caspase inhibitor (zVAD) was given in the treatment group. At 72 h after reperfusion, caspase-3 and XIAP expression were present in multiple vulnerable brain regions, whereas caspase-1 was predominantly found in the CAI hippocampus. zVAD significantly reduced expression of caspases and XIAP and the number of ischemic neurons in the CAI hippocampus while neurological deficit scores were improved. We conclude that hypoxic-ischemia increases caspases-1 and-3, and XIAP expression. Treatment with zVAD significantly decreases caspase and XIAP expression in these brain regions and improves neurological outcome.

Original languageEnglish
Pages (from-to)3751-3754
Number of pages4
JournalNeuroReport
Volume12
Issue number17
StatePublished - Dec 4 2001

Fingerprint

Caspase 1
Caspases
Heart Arrest
Caspase 3
Ischemia
Hippocampus
Brain
Apoptosis Regulatory Proteins
Caspase Inhibitors
Reperfusion
Neurons

Keywords

  • Cardiac arrest
  • Caspase
  • Hypoxia
  • Ischemia

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Regulation of caspases and XIAP in the brain after asphyxial cardiac arrest in rats. / Katz, Laurence M.; Lotocki, George; Wang, Yuanfan; Kraydieh, Susan; Dalton Dietrich, W.; Keane, Robert.

In: NeuroReport, Vol. 12, No. 17, 04.12.2001, p. 3751-3754.

Research output: Contribution to journalArticle

Katz, LM, Lotocki, G, Wang, Y, Kraydieh, S, Dalton Dietrich, W & Keane, R 2001, 'Regulation of caspases and XIAP in the brain after asphyxial cardiac arrest in rats', NeuroReport, vol. 12, no. 17, pp. 3751-3754.
Katz, Laurence M. ; Lotocki, George ; Wang, Yuanfan ; Kraydieh, Susan ; Dalton Dietrich, W. ; Keane, Robert. / Regulation of caspases and XIAP in the brain after asphyxial cardiac arrest in rats. In: NeuroReport. 2001 ; Vol. 12, No. 17. pp. 3751-3754.
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