Regulation of B lymphocyte responses to IL-4 and IFN-γ by activation through Ly-6A/E molecules

E. K. Codias, T. R. Malek

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

The Ly-6 family of cell surface molecules has previously been shown to participate in T cell activation. We show that Ly-6A/E proteins also modulated the response of normal B lymphocytes in three separate in vitro assays. First, unfractionated or small resting B cells proliferated when cultured with IFN-γ, IL-4, and an anti-Ly-6A/E mAb. Second, this anti-Ly-6A/E mAb restored B cell proliferation responses that were inhibited when coculturing the B cells in IFN-γ, IL-4, and anti-IgM. Third, anti-Ly-6A/E specifically up-regulated the cell surface expression of its own Ag, and this response was dependent upon costimulation with IFN-γ. Mixing of T and B cells in culture suggested that T cells did not contribute substantially to the B cell proliferative response. Moreover, up-regulation of Ly-6A/E was observed for one B cell lymphoma, WEHI-231. Therefore, it appeared that modulation of B cell function by anti-Ly-6A/E was due to a direct effect of the mAb binding to the B cells. Taken together, these data suggest Ly-6A/E proteins are functional on B cells and may play a regulatory role in B cell activation.

Original languageEnglish (US)
Pages (from-to)2197-2204
Number of pages8
JournalJournal of Immunology
Volume144
Issue number6
StatePublished - 1990

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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