TY - JOUR
T1 - Regulation of Antibody Production by Helper T Cell Clones in Experimental Autoimmune Myasthenia Gravis Is Mediated by IL-4 and Antigen-Specific T Cell Factors
AU - Asthana, Deshratn
AU - Fujii, Yoshitaka
AU - Huston, Gail E.
AU - Lindstrom, Jon
PY - 1993/6
Y1 - 1993/6
N2 - Acetylcholine receptor (AChR)-specific rat T cell clones (CD4+, CD8-, and OX22-) were shown to secrete both Th1 and Th2 lymphokines, IL-2, IL-4, and IFN-γ following stimulation with AChR. These clones helped production of antibody to AChR by B cells which was found to be regulated primarily by IL- 4, not by IL-2, secreted by the T cells in response to AChR. Cell-free supernatants from some AChR-activated T cell clones led to production of low levels of antibody to AChR by B cell-enriched, AChR-primed lymph node cells. Supernatant-regulated help in antibody production by B cells was antigen specific, and antibodies to T cell receptors blocked the antigen-specific activity. Thus, supernatant-mediated help may not be due solely to IL-4, and other factors, possibly including some fragment of the T cell antigen receptor present in the supernatant, appear to contribute to helping antibody production by B cells.
AB - Acetylcholine receptor (AChR)-specific rat T cell clones (CD4+, CD8-, and OX22-) were shown to secrete both Th1 and Th2 lymphokines, IL-2, IL-4, and IFN-γ following stimulation with AChR. These clones helped production of antibody to AChR by B cells which was found to be regulated primarily by IL- 4, not by IL-2, secreted by the T cells in response to AChR. Cell-free supernatants from some AChR-activated T cell clones led to production of low levels of antibody to AChR by B cell-enriched, AChR-primed lymph node cells. Supernatant-regulated help in antibody production by B cells was antigen specific, and antibodies to T cell receptors blocked the antigen-specific activity. Thus, supernatant-mediated help may not be due solely to IL-4, and other factors, possibly including some fragment of the T cell antigen receptor present in the supernatant, appear to contribute to helping antibody production by B cells.
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U2 - 10.1006/clin.1993.1071
DO - 10.1006/clin.1993.1071
M3 - Article
C2 - 7684661
AN - SCOPUS:0027190533
VL - 67
SP - 240
EP - 248
JO - Clinical Immunology
JF - Clinical Immunology
SN - 1521-6616
IS - 3
ER -