Secreted A β is produced during normal cell metabolism and it is regulated by many factors. Quantification of A β generation is an important approach to studying APP processing. In this chapter, we demonstrate that lithium, a potent GSK-3 inhibitor, can regulate amyloid secretion in COS7 cells overexpressing APPC100 demonstrated by a sandwich enzyme-linked immunosorbent assay (ELISA). Finally, we show that lithium can effectively inhibit tau pathology induced by A β injection into the hippocampus of a transgenic mouse model.
|Original language||English (US)|
|Title of host publication||Amyloid Precursor Protein|
|Subtitle of host publication||A Practical Approach|
|Number of pages||9|
|State||Published - Jan 1 2004|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)