Regulation of acetylcholine synthesis

Control of choline transport and acetylation in synaptosomes

Richard S Jope, M. H. Weiler, D. J. Jenden

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The high affinity transport of choline (Ch) and the synthesis of acetylcholine (ACh) were measured in synaptosomes by measuring the utilization of [2H4]Ch. The synthesis of ACh was reduced under several conditions which reduce the availability of acetyl coenzyme A (AcCoA) including no glucose added, replacement of glucose with succinate or impairment of glucose utilization by bromopyruvate, NaCN, or pentobarbital. These conditions did not reduce the amount of unacetylated [2H4]Ch in the synaptosomes indicating that the high affinity transport of Ch is not directly coupled to the synthesis of ACh.

Original languageEnglish
Pages (from-to)949-954
Number of pages6
JournalJournal of Neurochemistry
Volume30
Issue number5
StatePublished - Dec 1 1978
Externally publishedYes

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Acetylation
Synaptosomes
Choline
Acetylcholine
Glucose
Acetyl Coenzyme A
Succinic Acid
Pentobarbital
Availability

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Regulation of acetylcholine synthesis : Control of choline transport and acetylation in synaptosomes. / Jope, Richard S; Weiler, M. H.; Jenden, D. J.

In: Journal of Neurochemistry, Vol. 30, No. 5, 01.12.1978, p. 949-954.

Research output: Contribution to journalArticle

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