Regulation of a formin complex by the microtubule plus end protein tea1p

Becket Feierbach, Fulvia Verde, Fred Chang

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

The plus ends of microtubules have been speculated to regulate the actin cytoskeleton for the proper positioning of sites of cell polarization and cytokinesis. In the fission yeast Schizosaccharomyces pombe, interphase microtubules and the kelch repeat protein tea1p regulate polarized cell growth. Here, we show that tea1p is directly deposited at cell tips by microtubule plus ends. Tea1p associates in large "polarisome" complexes with bud6p and for3p, a formin that assembles actin cables. Tea1p also interacts in a separate complex with the CLIP-170 protein tip1p, a microtubule plus end-binding protein that anchors tea1p to the microtubule plus end. Localization experiments suggest that tea1p and bud6p regulate formin distribution and actin cable assembly. Although single mutants still polarize, for3Δbud6Δtea1Δ triple-mutant cells lack polarity, indicating that these proteins contribute overlapping functions in cell polarization. Thus, these experiments begin to elucidate how microtubules contribute to the proper spatial regulation of actin assembly and polarized cell growth.

Original languageEnglish
Pages (from-to)697-707
Number of pages11
JournalJournal of Cell Biology
Volume165
Issue number5
DOIs
StatePublished - Jun 7 2004

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Microtubules
Actins
Proteins
Schizosaccharomyces
Cell Polarity
Cytokinesis
Interphase
Growth
Actin Cytoskeleton
Carrier Proteins

Keywords

  • Actin
  • Cell polarity
  • Fission yeast
  • Formin
  • Microtubules

ASJC Scopus subject areas

  • Cell Biology

Cite this

Regulation of a formin complex by the microtubule plus end protein tea1p. / Feierbach, Becket; Verde, Fulvia; Chang, Fred.

In: Journal of Cell Biology, Vol. 165, No. 5, 07.06.2004, p. 697-707.

Research output: Contribution to journalArticle

Feierbach, Becket ; Verde, Fulvia ; Chang, Fred. / Regulation of a formin complex by the microtubule plus end protein tea1p. In: Journal of Cell Biology. 2004 ; Vol. 165, No. 5. pp. 697-707.
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