Regulation and selective expression of Ly-6A/E, a lymphocyte activation molecule, in the central nervous system

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Abstract

The Ly-6 locus encodes a group of cell surface molecules found predominately on lymphoid cells in the mouse. These proteins share several structural and functional characteristics with Thy-1, a molecule expressed in both lymphoid and neuronal tissue. Utilizing anti-Ly-6A/E monoclonal antibodies, the present results demonstrate in situ expression of these molecules in brain tissue. The findings also indicated that these molecules are not expressed during embryonic or neonatal stages of development. Moreover, although Ly-6b haplotype mice exhibited staining primary associated with vascular elements throughout the brain, Ly-6a mice exhibited staining predominantly associated with white matter limited to the hippocampal and midbrain regions. Although cultured glial and neuronal cells expressed marginally detectable levels of Ly-6A/E, the majority of GFAP+ cells in these cultures expressed high levels of Ly-6A/E following incubation with cytokines including rIFN-gamma. In addition, northern blot analysis of RNA from enriched astrocytic cultures corroborated the induction of Ly-6A/E expression. These findings have therefore established that Ly-6 is amongst those groups of genes expressed in both brain and lymphoid tissues.

Original languageEnglish
Pages (from-to)9-15
Number of pages7
JournalMolecular Brain Research
Volume8
Issue number1
DOIs
StatePublished - Jan 1 1990

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Lymphocyte Activation
Central Nervous System
Lymphoid Tissue
Brain
Staining and Labeling
Mesencephalon
Neuroglia
Northern Blotting
Haplotypes
Blood Vessels
Cultured Cells
Cell Culture Techniques
Monoclonal Antibodies
Lymphocytes
RNA
Cytokines
Genes
Proteins

Keywords

  • Astrocyte
  • Cytokine
  • Interferon-γ
  • Ly-6A/E
  • Murine brain
  • Thy-1

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

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title = "Regulation and selective expression of Ly-6A/E, a lymphocyte activation molecule, in the central nervous system",
abstract = "The Ly-6 locus encodes a group of cell surface molecules found predominately on lymphoid cells in the mouse. These proteins share several structural and functional characteristics with Thy-1, a molecule expressed in both lymphoid and neuronal tissue. Utilizing anti-Ly-6A/E monoclonal antibodies, the present results demonstrate in situ expression of these molecules in brain tissue. The findings also indicated that these molecules are not expressed during embryonic or neonatal stages of development. Moreover, although Ly-6b haplotype mice exhibited staining primary associated with vascular elements throughout the brain, Ly-6a mice exhibited staining predominantly associated with white matter limited to the hippocampal and midbrain regions. Although cultured glial and neuronal cells expressed marginally detectable levels of Ly-6A/E, the majority of GFAP+ cells in these cultures expressed high levels of Ly-6A/E following incubation with cytokines including rIFN-gamma. In addition, northern blot analysis of RNA from enriched astrocytic cultures corroborated the induction of Ly-6A/E expression. These findings have therefore established that Ly-6 is amongst those groups of genes expressed in both brain and lymphoid tissues.",
keywords = "Astrocyte, Cytokine, Interferon-γ, Ly-6A/E, Murine brain, Thy-1",
author = "Carolyn Cray and Robert Keane and Thomas Malek and Levy, {Robert B}",
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T1 - Regulation and selective expression of Ly-6A/E, a lymphocyte activation molecule, in the central nervous system

AU - Cray, Carolyn

AU - Keane, Robert

AU - Malek, Thomas

AU - Levy, Robert B

PY - 1990/1/1

Y1 - 1990/1/1

N2 - The Ly-6 locus encodes a group of cell surface molecules found predominately on lymphoid cells in the mouse. These proteins share several structural and functional characteristics with Thy-1, a molecule expressed in both lymphoid and neuronal tissue. Utilizing anti-Ly-6A/E monoclonal antibodies, the present results demonstrate in situ expression of these molecules in brain tissue. The findings also indicated that these molecules are not expressed during embryonic or neonatal stages of development. Moreover, although Ly-6b haplotype mice exhibited staining primary associated with vascular elements throughout the brain, Ly-6a mice exhibited staining predominantly associated with white matter limited to the hippocampal and midbrain regions. Although cultured glial and neuronal cells expressed marginally detectable levels of Ly-6A/E, the majority of GFAP+ cells in these cultures expressed high levels of Ly-6A/E following incubation with cytokines including rIFN-gamma. In addition, northern blot analysis of RNA from enriched astrocytic cultures corroborated the induction of Ly-6A/E expression. These findings have therefore established that Ly-6 is amongst those groups of genes expressed in both brain and lymphoid tissues.

AB - The Ly-6 locus encodes a group of cell surface molecules found predominately on lymphoid cells in the mouse. These proteins share several structural and functional characteristics with Thy-1, a molecule expressed in both lymphoid and neuronal tissue. Utilizing anti-Ly-6A/E monoclonal antibodies, the present results demonstrate in situ expression of these molecules in brain tissue. The findings also indicated that these molecules are not expressed during embryonic or neonatal stages of development. Moreover, although Ly-6b haplotype mice exhibited staining primary associated with vascular elements throughout the brain, Ly-6a mice exhibited staining predominantly associated with white matter limited to the hippocampal and midbrain regions. Although cultured glial and neuronal cells expressed marginally detectable levels of Ly-6A/E, the majority of GFAP+ cells in these cultures expressed high levels of Ly-6A/E following incubation with cytokines including rIFN-gamma. In addition, northern blot analysis of RNA from enriched astrocytic cultures corroborated the induction of Ly-6A/E expression. These findings have therefore established that Ly-6 is amongst those groups of genes expressed in both brain and lymphoid tissues.

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