Abstract
The tissue-specific transcription factor GATA-1 is a key regulator of red blood cell differentiation. One seemingly contradictory aspect of GATA-1 function is that, while it is abundant in erythroid progenitor cells prior to the onset of overt differentiation, it does not significantly activate known GATA-1 target genes in those cells. To investigate the mechanisms underlying GATA-1 function during the transition from early to late erythropoiesis, we have examined its expression and activity in normal avian erythroid progenitor cells before and after induction of differentiation. In these primary progenitor cells, GATA-1 protein was predominantly located in the cytoplasm, while induction of differentiation caused its rapid relocalization to the nucleus, suggesting that nuclear translocation constitutes an important regulatory step in GATA-1 activation. As an alternative way of addressing the same question, we also ectopically expressed a GATA-1/estrogen receptor fusion protein (GATA-1/ER) in red blood cell progenitors, where nuclear translocation of, and transcriptional activation by, this hybrid factor are conditionally controlled by estrogen. We found that hormone-activated GATA-1/ER protein accelerated red blood cell differentiation, and concomitantly suppressed cell proliferation. These phenotypic effects were accompanied by a simultaneous suppression of c-myb and GATA-2 transcription, two genes thought to be involved in the proliferative capacity of hematopoietic progenitor cells. Thus, GATA-1 appears to promote differentiation in committed erythroid progenitor cells both by inducing differentiation-specific genes and by simultaneously suppressing genes involved in cell proliferation.
| Original language | English |
|---|---|
| Pages (from-to) | 3839-3850 |
| Number of pages | 12 |
| Journal | Development |
| Volume | 122 |
| Issue number | 12 |
| State | Published - Dec 1 1996 |
| Externally published | Yes |
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Keywords
- Erythroid differentiation
- GATA-1 transcription factor
- Nuclear translocation
- Phosphorylation
- Red blood cell
- Transcription factor
ASJC Scopus subject areas
- Anatomy
- Cell Biology
Cite this
Regulation and function of transcription factor GATA-1 during red blood cell differentiation. / Briegel, Karoline; Bartunek, Petr; Stengl, Gabi; Lim, Kim Chew; Beug, Hartmut; Engel, James Douglas; Zenke, Martin.
In: Development, Vol. 122, No. 12, 01.12.1996, p. 3839-3850.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Regulation and function of transcription factor GATA-1 during red blood cell differentiation
AU - Briegel, Karoline
AU - Bartunek, Petr
AU - Stengl, Gabi
AU - Lim, Kim Chew
AU - Beug, Hartmut
AU - Engel, James Douglas
AU - Zenke, Martin
PY - 1996/12/1
Y1 - 1996/12/1
N2 - The tissue-specific transcription factor GATA-1 is a key regulator of red blood cell differentiation. One seemingly contradictory aspect of GATA-1 function is that, while it is abundant in erythroid progenitor cells prior to the onset of overt differentiation, it does not significantly activate known GATA-1 target genes in those cells. To investigate the mechanisms underlying GATA-1 function during the transition from early to late erythropoiesis, we have examined its expression and activity in normal avian erythroid progenitor cells before and after induction of differentiation. In these primary progenitor cells, GATA-1 protein was predominantly located in the cytoplasm, while induction of differentiation caused its rapid relocalization to the nucleus, suggesting that nuclear translocation constitutes an important regulatory step in GATA-1 activation. As an alternative way of addressing the same question, we also ectopically expressed a GATA-1/estrogen receptor fusion protein (GATA-1/ER) in red blood cell progenitors, where nuclear translocation of, and transcriptional activation by, this hybrid factor are conditionally controlled by estrogen. We found that hormone-activated GATA-1/ER protein accelerated red blood cell differentiation, and concomitantly suppressed cell proliferation. These phenotypic effects were accompanied by a simultaneous suppression of c-myb and GATA-2 transcription, two genes thought to be involved in the proliferative capacity of hematopoietic progenitor cells. Thus, GATA-1 appears to promote differentiation in committed erythroid progenitor cells both by inducing differentiation-specific genes and by simultaneously suppressing genes involved in cell proliferation.
AB - The tissue-specific transcription factor GATA-1 is a key regulator of red blood cell differentiation. One seemingly contradictory aspect of GATA-1 function is that, while it is abundant in erythroid progenitor cells prior to the onset of overt differentiation, it does not significantly activate known GATA-1 target genes in those cells. To investigate the mechanisms underlying GATA-1 function during the transition from early to late erythropoiesis, we have examined its expression and activity in normal avian erythroid progenitor cells before and after induction of differentiation. In these primary progenitor cells, GATA-1 protein was predominantly located in the cytoplasm, while induction of differentiation caused its rapid relocalization to the nucleus, suggesting that nuclear translocation constitutes an important regulatory step in GATA-1 activation. As an alternative way of addressing the same question, we also ectopically expressed a GATA-1/estrogen receptor fusion protein (GATA-1/ER) in red blood cell progenitors, where nuclear translocation of, and transcriptional activation by, this hybrid factor are conditionally controlled by estrogen. We found that hormone-activated GATA-1/ER protein accelerated red blood cell differentiation, and concomitantly suppressed cell proliferation. These phenotypic effects were accompanied by a simultaneous suppression of c-myb and GATA-2 transcription, two genes thought to be involved in the proliferative capacity of hematopoietic progenitor cells. Thus, GATA-1 appears to promote differentiation in committed erythroid progenitor cells both by inducing differentiation-specific genes and by simultaneously suppressing genes involved in cell proliferation.
KW - Erythroid differentiation
KW - GATA-1 transcription factor
KW - Nuclear translocation
KW - Phosphorylation
KW - Red blood cell
KW - Transcription factor
UR - http://www.scopus.com/inward/record.url?scp=0030481726&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030481726&partnerID=8YFLogxK
M3 - Article
C2 - 9012505
AN - SCOPUS:0030481726
VL - 122
SP - 3839
EP - 3850
JO - Development (Cambridge)
JF - Development (Cambridge)
SN - 0950-1991
IS - 12
ER -