Regional increase in isolated myocyte volume in chronic myocardial infarction in cats

P. L. Kozlovskis, A. M. Gerdes, M. Smets, J. A. Moore, A. L. Bassett, Robert J Myerburg

Research output: Contribution to journalArticle

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Abstract

Healing of myocardial infarction is associated with hypertrophy of a region surrounding the scar. In order to characterize the pattern of regional hypertrophy after healing of small myocardial infarctions, we used a Coulter Channelyzer to measure directly regional cell volume and light microscopy to measure cell length of isolated myocytes. Acute left ventricular myocardial infarctions were surgically created in adult cat hearts. After healing for 10.4 ± 5.0 months, cells were dissociated by collagenase perfusion. Myocardial cells were isolated from three regions of the infarcted ventricle and the same three anatomical regions of un-operated control hearts: (1) remote from the infarct, (2) non-scarred tissues adjacent to the infarct, and (3) from the infarct. The volume of cells from control hearts was correlated significantly with individual body weight resulting in large inter-animal variations, but small intra-animal variations. Inter-animal comparisons were made by normalizing adjacent and infarct regions to percent change from its remote region. Myocyte volumes from hearts with healed infarcts were increased by 31% in the infarct region and by 20% in the adjacent region, relative to the corresponding regions from control hearts (P<0.05). Cell lengths were not different from control in any region. Calculated cross-sectional areas followed the same pattern as was observed for cell volumes. We conclude that there is a region of hypertrophy surrounding a small, transmural healed myocardial infarction that is characterized by increased myocyte cross sectional area with no change in cell length. This pattern is typical of the concentric hypertrophy observed with pressure overload rather than eccentric hypertrophy observed with volume overload.

Original languageEnglish
Pages (from-to)1459-1466
Number of pages8
JournalJournal of Molecular and Cellular Cardiology
Volume23
Issue number12
DOIs
StatePublished - Jan 1 1991

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Muscle Cells
Hypertrophy
Cats
Myocardial Infarction
Cell Size
Cardiac Volume
Collagenases
Cicatrix
Microscopy
Perfusion
Body Weight
Light
Pressure

Keywords

  • Hypertrophy
  • Morphometry
  • Myocardial infarction

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Regional increase in isolated myocyte volume in chronic myocardial infarction in cats. / Kozlovskis, P. L.; Gerdes, A. M.; Smets, M.; Moore, J. A.; Bassett, A. L.; Myerburg, Robert J.

In: Journal of Molecular and Cellular Cardiology, Vol. 23, No. 12, 01.01.1991, p. 1459-1466.

Research output: Contribution to journalArticle

Kozlovskis, P. L. ; Gerdes, A. M. ; Smets, M. ; Moore, J. A. ; Bassett, A. L. ; Myerburg, Robert J. / Regional increase in isolated myocyte volume in chronic myocardial infarction in cats. In: Journal of Molecular and Cellular Cardiology. 1991 ; Vol. 23, No. 12. pp. 1459-1466.
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abstract = "Healing of myocardial infarction is associated with hypertrophy of a region surrounding the scar. In order to characterize the pattern of regional hypertrophy after healing of small myocardial infarctions, we used a Coulter Channelyzer to measure directly regional cell volume and light microscopy to measure cell length of isolated myocytes. Acute left ventricular myocardial infarctions were surgically created in adult cat hearts. After healing for 10.4 ± 5.0 months, cells were dissociated by collagenase perfusion. Myocardial cells were isolated from three regions of the infarcted ventricle and the same three anatomical regions of un-operated control hearts: (1) remote from the infarct, (2) non-scarred tissues adjacent to the infarct, and (3) from the infarct. The volume of cells from control hearts was correlated significantly with individual body weight resulting in large inter-animal variations, but small intra-animal variations. Inter-animal comparisons were made by normalizing adjacent and infarct regions to percent change from its remote region. Myocyte volumes from hearts with healed infarcts were increased by 31{\%} in the infarct region and by 20{\%} in the adjacent region, relative to the corresponding regions from control hearts (P<0.05). Cell lengths were not different from control in any region. Calculated cross-sectional areas followed the same pattern as was observed for cell volumes. We conclude that there is a region of hypertrophy surrounding a small, transmural healed myocardial infarction that is characterized by increased myocyte cross sectional area with no change in cell length. This pattern is typical of the concentric hypertrophy observed with pressure overload rather than eccentric hypertrophy observed with volume overload.",
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