Regional glucose utilization and blood flow following graded forebrain ischemia in the rat

Correlation with neuropathology

Myron Ginsberg, D. I. Graham, R. Busto

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Regional patterns of cerebral glucose utilization (rCMRglc) and blood flow (rCBF) were examined in the early recovery period following transient forebrain ischemia in order to correlate early postischemic physiological events with regionally selective patterns of ischemic neuropathology. Wistar rats were subjected to 30 or 60 minutes of graded forebrain ischemia by a method combining unilateral occlusion of the common carotid artery with moderate elevation of intracranial pressure and mild hypotension; this procedure results in a high-grade ischemic deficit affecting chiefly the lateral neocortex, striatum, and hippocampus ipsilateral to the carotid occlusion. Simultaneous measurements of rCMRglc and rCBF made in regional tissue samples after 2 and 4 hours of postischemic recirculation using a double-tracer radioisotopic strategy revealed a disproportionately high level of glucose metabolism relative to blood flow in the early postischemic striatum, owing to the resumption of nearly normal rCMRglc in the face of depressed flow. In contrast, the neocortex, which had been equally ischemic, showed parallel depressions of both metabolism and blood flow during early recovery. Light microscopy at 4 and 8 hours after recovery revealed the striatum to be the predominant locus of ischemic neuronal alterations, whereas neocortical lesions were much less prominent in extent and severity at this time. The resumption of normal levels of metabolism in the setting of a disproportionate depression of rCBF in the early postischemic period may accentuate the process of neuronal injury initiated by ischemia and may contribute to the genesis of neuronal necrosis in 'selectively vulnerable' areas of the forebrain.

Original languageEnglish
Pages (from-to)470-481
Number of pages12
JournalAnnals of Neurology
Volume18
Issue number4
StatePublished - Dec 1 1985

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Prosencephalon
Blood Glucose
Ischemia
Neocortex
Glucose
Intracranial Hypertension
Common Carotid Artery
Hypotension
Wistar Rats
Microscopy
Hippocampus
Necrosis
Neuropathology
Light
Wounds and Injuries

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Regional glucose utilization and blood flow following graded forebrain ischemia in the rat : Correlation with neuropathology. / Ginsberg, Myron; Graham, D. I.; Busto, R.

In: Annals of Neurology, Vol. 18, No. 4, 01.12.1985, p. 470-481.

Research output: Contribution to journalArticle

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