Regional distribution of maternal messenger RNA in the amphibian oocyte

Mary Lou L King, Eric Barklis

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

We show that there is a non-random distribution of information in the form of mRNAs along the animal/vegetal axis of Xenopus oocytes. Polyadenylated RNA isolated from different regions along this axis was translated in a cell-free system, and the products were fractionated by two-dimensional gel electrophoresis. A comparison of the resulting protein products using a computer program has established that specific translatable mRNAs are regionally localized within the oocyte prior to fertilization. Seventeen mRNAs show significant qualitative or quantitative differences in their subcellular location. These sequences comprise as much as 4% of the total detected translatable polyadenylated RNA. Asymmetric segregation of these messages during Xenopus development may be involved in the determination of cell fate.

Original languageEnglish
Pages (from-to)203-212
Number of pages10
JournalDevelopmental Biology
Volume112
Issue number1
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

Fingerprint

Stored Messenger RNA
Amphibians
Oocytes
Messenger RNA
Xenopus
Cell-Free System
Information Dissemination
Electrophoresis, Gel, Two-Dimensional
Fertilization
Software
Proteins

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Regional distribution of maternal messenger RNA in the amphibian oocyte. / King, Mary Lou L; Barklis, Eric.

In: Developmental Biology, Vol. 112, No. 1, 01.01.1985, p. 203-212.

Research output: Contribution to journalArticle

King, Mary Lou L ; Barklis, Eric. / Regional distribution of maternal messenger RNA in the amphibian oocyte. In: Developmental Biology. 1985 ; Vol. 112, No. 1. pp. 203-212.
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