Regional Differential Genetic Response of Human Articular Cartilage to Impact Injury

Lauren L. Vernon, Danica D. Vance, Liyong Wang, Evadnie Rampersaud, Jeffery M. Vance, Margaret Pericak-Vance, C. Y.Charles Huang, Lee D. Kaplan

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Objective: Normal physiological movement creates different weightbearing zones within a human knee: the medial condyle bearing the highest and the trochlea bearing the lowest weight. Adaptation to different physiological loading conditions results in different tissue and cellular properties within a knee. The objective of this study was to use microarray analysis to examine gene expression differences among three anatomical regions of human knee articular cartilage at baseline and following induction of an acute impact injury. Design: Cartilage explants were harvested from 7 cadaveric knees (12 plugs per knee). A drop tower was utilized to introduce injury. Plugs were examined 24 hours after impact for gene expression using microarray. The primary analysis is the comparison of baseline versus impacted samples within each region separately. In addition, pairwise comparisons among the three regions were performed at baseline and after impact. False discovery rate (FDR) was used to evaluate significance of differential gene expression. Results: In the comparison of before and after injury, the trochlear had 130 differentially expressed genes (FDR ≤ 0.05) while the condyles had none. In the comparison among regions, smaller sets of differentially expressed genes (n ≤ 21) were found, with trochlea being more different than the condyles. Most of more frequently expressed genes in trochlea are developmental genes. Conclusions: Within the experimental setup of this study, only the trochlea was displaying an acute genetic response on injury. Our data demonstrated the regional-specific response to injury in human articular cartilage.

Original languageEnglish (US)
Pages (from-to)163-173
Number of pages11
JournalCartilage
Volume7
Issue number2
DOIs
StatePublished - Apr 1 2016

Keywords

  • articular cartilage
  • human knee
  • impact injury
  • regional genetic expression

ASJC Scopus subject areas

  • Immunology and Allergy
  • Biomedical Engineering
  • Physical Therapy, Sports Therapy and Rehabilitation

Fingerprint Dive into the research topics of 'Regional Differential Genetic Response of Human Articular Cartilage to Impact Injury'. Together they form a unique fingerprint.

  • Cite this