Regional Differences in Islet Distribution in the Human Pancreas - Preferential Beta-Cell Loss in the Head Region in Patients with Type 2 Diabetes

Xiaojun Wang, Ryosuke Misawa, Mark C. Zielinski, Peter Cowen, Junghyo Jo, Vipul Periwal, Camillo Ricordi, Aisha Khan, Joel Szust, Junhui Shen, J. Michael Millis, Piotr Witkowski, Manami Hara

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

While regional heterogeneity in islet distribution has been well studied in rodents, less is known about human pancreatic histology. To fill gaps in our understanding, regional differences in the adult human pancreas were quantitatively analyzed including the pathogenesis of type 2 diabetes (T2D). Cadaveric pancreas specimens were collected from the head, body and tail regions of each donor, including subjects with no history of diabetes or pancreatic diseases (n = 23) as well as patients with T2D (n = 12). The study further included individuals from whom islets were isolated (n = 7) to study islet yield and function in a clinical setting of islet transplantation. The whole pancreatic sections were examined using an innovative large-scale image capture and unbiased detailed quantitative analyses of the characteristics of islets from each individual (architecture, size, shape and distribution). Islet distribution/density is similar between the head and body regions, but is >2-fold higher in the tail region. In contrast to rodents, islet cellular composition and architecture were similar throughout the pancreas and there was no difference in glucose-stimulated insulin secretion in islets isolated from different regions of the pancreas. Further studies revealed preferential loss of large islets in the head region in patients with T2D. The present study has demonstrated distinct characteristics of the human pancreas, which should provide a baseline for the future studies integrating existing research in the field and helping to advance bi-directional research between humans and preclinical models.

Original languageEnglish
Article numbere67454
JournalPLoS One
Volume8
Issue number6
DOIs
StatePublished - Jun 24 2013

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Medical problems
noninsulin-dependent diabetes mellitus
pancreas
Type 2 Diabetes Mellitus
Pancreas
Head
Body Regions
cells
Tail
Rodentia
rodents
tail
Histology
pancreatic diseases
Islets of Langerhans Transplantation
Pancreatic Diseases
body regions
insulin secretion
Research
History

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Regional Differences in Islet Distribution in the Human Pancreas - Preferential Beta-Cell Loss in the Head Region in Patients with Type 2 Diabetes. / Wang, Xiaojun; Misawa, Ryosuke; Zielinski, Mark C.; Cowen, Peter; Jo, Junghyo; Periwal, Vipul; Ricordi, Camillo; Khan, Aisha; Szust, Joel; Shen, Junhui; Millis, J. Michael; Witkowski, Piotr; Hara, Manami.

In: PLoS One, Vol. 8, No. 6, e67454, 24.06.2013.

Research output: Contribution to journalArticle

Wang, X, Misawa, R, Zielinski, MC, Cowen, P, Jo, J, Periwal, V, Ricordi, C, Khan, A, Szust, J, Shen, J, Millis, JM, Witkowski, P & Hara, M 2013, 'Regional Differences in Islet Distribution in the Human Pancreas - Preferential Beta-Cell Loss in the Head Region in Patients with Type 2 Diabetes', PLoS One, vol. 8, no. 6, e67454. https://doi.org/10.1371/journal.pone.0067454
Wang, Xiaojun ; Misawa, Ryosuke ; Zielinski, Mark C. ; Cowen, Peter ; Jo, Junghyo ; Periwal, Vipul ; Ricordi, Camillo ; Khan, Aisha ; Szust, Joel ; Shen, Junhui ; Millis, J. Michael ; Witkowski, Piotr ; Hara, Manami. / Regional Differences in Islet Distribution in the Human Pancreas - Preferential Beta-Cell Loss in the Head Region in Patients with Type 2 Diabetes. In: PLoS One. 2013 ; Vol. 8, No. 6.
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