Regional changes in intracellular potassium and sodium activity after healing of experimental myocardial infarction in cats

S. Kimura, A. L. Bassett, M. S. Gaide, P. L. Kozlovskis, R. J. Myerburg

Research output: Contribution to journalArticle

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Abstract

After healing of experimental myocardial infarction in cat hearts, endocardial cells demonstrate persistent regional electrical changes. These include long action potential duration in surviving cells over the infarct scar, and short action potential duration and low membrane potential in border zone cells between the scar and normal tissue. We studied the basis for these electrophysiological changes by measuring intracellular potassium and sodium activity with ionsensitive microelectrodes in normal, border, and infarct zone cells of the cat left ventricle 2-6 months after ligation of multiple distal tributaries of the left anterior descending and circumflex coronary arteries. In normal zone cells, intracellular potassium activity was 89.6 ± 12.3 mM (mean ± SD, n = 9), and sodium activity was 10.8 ± 2.2 mM (n = 5). Neither was significantly different from infarct zone cells (91.2 ± 15.0 and 10.5 ± 3.0 mM, respectively). In contrast, border zone cells had significantly reduced intracellular potassium activity (71.4 ± 5.1 mM, P < 0.01, compared to normal and infarct zone cells) and increased intracellular sodium activity (19.1 ± 5.9 mM, P < 0.05, compared to normal and infarct zone cells). The membrane potential in border zone cells was more positive to calculated potassium equilibrium potential, and less sensitive to the change in the extracellular potassium concentration in the range between 2 and 10 mM, compared to normal and infarct zone cells. Sixty minutes of exposure to 5 x 10-7 M ouabain reduced the membrane potential and intracellular potassium activity to a lesser degree in border zone cells than in normal and infarct zone cells (P < 0.05), but the percent changes from the initial values were not significantly different among the three zone cells. We conclude that action potential changes in infarct zone cells are not accompanied by changes in intracellular potassium and sodium activities, while border zone cell changes are accompanied by reduced intracellular potassium activity and increased intracellular sodium activity. Our data also suggest that border zone cells have altered potassium and/or sodium conductances.

Original languageEnglish (US)
Pages (from-to)202-208
Number of pages7
JournalCirculation research
Volume58
Issue number2
DOIs
StatePublished - Jan 1 1986

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ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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