Redundant ubiquitin ligase activities regulate wee1 degradation and mitotic entry

Anthony Smith, Scott Simanski, Mohammad Fallahi, Nagi Ayad

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The irreversible nature of mitotic entry is due to the activation of mitosis specific kinases such as cdk1/cyclin B. Cdk1/cyclin B induces activation of mitosis by promoting phosphatases while suppressing inhibitory factors such as the tyrosine kinase wee1. Since wee1 inactivates cdk1/cyclin B during the S and G2 phases, its activity must be downregulated for mitotic progression to occur. One mechanism of suppressing wee1 activity is ubiquitin-dependent proteolysis. Cdk1/cyclin B1 phosphorylates wee1, targeting it for recognition by ubiquitin ligases and subsequent proteasomal degradation. One of the ubiquitin ligases promoting wee1 destruction during mitosis is the SCFβ-trcp complex. We demonstrate that this complex, and a second SCF complex containing the F-box protein Tome-1, regulate wee1 degradation during the S and G2 phases of the cell cycle. Therefore, redundant ubiquitin ligase activities promote efficient mitotic entry of eukaryotic cells.

Original languageEnglish
Pages (from-to)2795-2799
Number of pages5
JournalCell Cycle
Volume6
Issue number22
StatePublished - Nov 15 2007
Externally publishedYes

Fingerprint

Ligases
Ubiquitin
Cyclin B
Mitosis
Degradation
G2 Phase
S Phase
Chemical activation
F-Box Proteins
Proteolysis
Cyclin B1
Eukaryotic Cells
Phosphoric Monoester Hydrolases
Protein-Tyrosine Kinases
Cell Cycle
Phosphotransferases
Down-Regulation
Cells

Keywords

  • β-TRCP
  • Degradation
  • F-box
  • Mitosis
  • Tome-1
  • Wee1

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Cite this

Redundant ubiquitin ligase activities regulate wee1 degradation and mitotic entry. / Smith, Anthony; Simanski, Scott; Fallahi, Mohammad; Ayad, Nagi.

In: Cell Cycle, Vol. 6, No. 22, 15.11.2007, p. 2795-2799.

Research output: Contribution to journalArticle

Smith, A, Simanski, S, Fallahi, M & Ayad, N 2007, 'Redundant ubiquitin ligase activities regulate wee1 degradation and mitotic entry', Cell Cycle, vol. 6, no. 22, pp. 2795-2799.
Smith, Anthony ; Simanski, Scott ; Fallahi, Mohammad ; Ayad, Nagi. / Redundant ubiquitin ligase activities regulate wee1 degradation and mitotic entry. In: Cell Cycle. 2007 ; Vol. 6, No. 22. pp. 2795-2799.
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